Although the fluorescent dye detached from the degraded POL - N probe stayed for some time in normoxic cells, the intact POL - N probe rapidly disappeared from the circulation and excretory organs, including the kidney and liver, dramatically reducing off - target BRET signals because they are generated from only intact POL - N, and thus increasing tumour / muscle and tumour / kidney signal ratios (Figure c). (scicasts.com)
Of the 119 genes upregulated following hypoxia, 72 % remained upregulated after a 24 h period of recovery under normoxic conditions. (molvis.org)
In normoxic cells, the transcription factor HIF - 1α is degraded by the action of E3 ubiquitin ligase on the oxygen - dependent degradation domain (ODD) of HIF - 1α; however HIF - 1α is stabilized and therefore, accumulates in hypoxic cancer cells. (scicasts.com)