Following introduction into human CD4 + T cells [23] or hematopoietic stem cells [28] via an adenovirus vector or DNA nucleofection, respectively, the ccr5 gene was efficiently and specifically disrupted. (journals.plos.org)
We prefer to culture our cells on mouse embryo fibroblasts (MEFs) but occasionally grow the cells feeder free for particular applications, such as nucleofection, viral transduction or karyotyping. (stembook.org)
For every one genetically altered cell generated using the chemical method, the new growth factor / nucleofection method produces between 10 and 100 successfully modified cells, UCI scientists estimate. (sciencedaily.com)