Dr. Lakadamyali states that «at the moment we have to work with imperfect fluorescent proteins and take into account the limitations when quantifying protein stoichiometry, however, now that there is a robust way to characterize photoactivation efficiency, future fluorescent protein engineering work will likely focus on optimizing this parameter for generating fluorescent proteins better suited for molecular counting using super-resolution». (sciencedaily.com)
Nguyen D, Mohan M, Elsässer SJ, Uttamapinant C, Chin JW, «Genetic encoding of photocaged cysteine allows photoactivation of TEV protease in live mammalian cells ``, JACS (2014) (scilifelab.se)
Instead, the new method, called patterned photoactivation non-linear SIM, begins by switching on just a subset of fluorescent labels in a sample with a pattern of light. (sciencedaily.com)