Multiple, overlapping amplifications with different primer pairs were used to confirm all SNPs. (journals.plos.org)
Sequence analysis of the 283 bp crd3 - affected amplicon from primer pair 7 showed that exons 15 and 16 are absent from affected cDNA. (molvis.org)
Subsequently, amplification with primer pair 7 (exon 14 to 18) gave 573 bp and 283 bp product from normal and affected dogs, respectively. (molvis.org)