Quantitative PCR reactions were performed using primer sets corresponding to murine JAK1 or murine Actin with qPCR ™ Mastermix for SYBR Green I (Eurogentec). (haematologica.org)
The authors of this study have developed a universal primer set across flowering plants that amplifies 3 - 15 kilobase fragments, which can then easily be sequenced using recently developed next - generation sequencing technologies. (sciencedaily.com)
The gene expression from every sample was amplified in triplicates, with a single volume of 10 µl containing: 3 µl of diluted cNDA with 0.5 µl of primer set (see Table 1 for primer sequences), 1.5 µl double distilled water (DDW) and 5 µl of GoTaq qpcr Master Mix Kit (Promega, USA). (peerj.com)