Once they'd found suitable pairs, they constructed three components: GBPa - VP16 (activation domain); GBPb - GAL4 (DNA - binding domain); and a UAS - driven luciferase reporter construct. (blog.addgene.org)
The mutant reporter constructs pXP2 - mut - 1, pXP2 - mut - 2, and pXP2 - mut - 3 were generated from pXP2 (− 310) by Mutagenesis kit. (cancerres.aacrjournals.org)
Although this problem can, to some extent, be addressed by the use of reporter constructs (e.g. Diptericin - LacZ) that provide quantitative or visual read - outs from specific immune response genes, we still lack the ability to follow bacterial infections in real - time in the critical early stages of infection. (journals.plos.org)