Aβ was immunoprecipitated from 50 μl CSF with 5 μg of the anti-Aβ antibody W0 - 2 (EMI Millipore, USA) coupled to protein - G sepharose beads (GE Healthcare, USA) and incubated overnight at 4 °C. (actaneurocomms.biomedcentral.com)
Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top). (cancerres.aacrjournals.org)
The next day, protein G sepharose was used to immunoprecipitate the RIPK2 protein complex immunoprecipitation (IP) for 1.5 hours. (jpet.aspetjournals.org)