The PCR product was cloned and the isolated Kpn I / Nhe I fragment then subcloned into a pBS sk - plasmid bearing a GFP (afmx) sequence with an Nhe I site at the translational start of GFP. (neuraldevelopment.biomedcentral.com)
Psin - miR7 - 2 plasmid was constructed by subcloning PCR product from pCDH - CMV - MCS - EF1 - copGFP - miR7 - 2. (cancerres.aacrjournals.org)
The cDNAs were then subcloned into the pCEP4β episomal plasmid (Invitrogen). (journals.plos.org)