The Morris lab has since determined the mechanistic underpinnings of non-coding RNA mediated regulation of gene transcription in human cells, with evidence suggesting a role for longer forms of non-coding RNAs as endogenous effectors involved in epigenetically remodelling target loci in human cells. (silence-therapeutics.com)
When coexpressed with a recombinase, these ssDNAs address specific target loci on the basis of sequence homology and introduce precise mutations into genomic DNA, thus converting transient cellular signals into genomically encoded memory. (science.sciencemag.org)
By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus. (science.sciencemag.org)