The phrase "toothpick test" means poking a toothpick or a similar object into food to check if it is cooked or baked properly. If the toothpick comes out clean, it means the food is cooked all the way through. Full definition
Bake 55 - 60 minutes or until toothpick tests clean. (eatpicks.com)
Mine tends toward 1 hour and 30 minutes for a successful toothpick test. (healthyflour.com)
(using toothpick testing) ovens can vary especially I find when baking at lower temperatures so the cupcakes could take anywhere from 40 - 60 minutes to bake. (artandthekitchen.com)