When primary cultures from these mice are stimulated with proinflammatory cytokines, the
endothelial cell fraction responds by up - regulating the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1.
Quantitative PCR analysis was performed to compare transcript levels of Venus with PrEn (Hex, Gata4, Gata6, Dab2, Sox7, Hnf4α, and Pdgfrα), pluripotency (Nanog, Klf4, Rex1, Stella, and Pou5f1) and other lineage (T, Fgf5, Eomes, Flk1, Mixl1, Cdx2, Sox1, Pax6, and Six3) markers in
purified cell fractions.