Sentences with word «congenic»

Lin C, Fesi BD, Marquis M, Bosak NP, Lysenko A, Koshnevisan MA, Duke FF, Theodorides ML, Nelson TM, McDaniel AH, Avigdor M, Arayata CJ, Shaw L, Bachmanov AA, Reed DR. Adiposity QTL Adip20 decomposes into at least four loci when dissected using congenic strains.

In contrast, the Speed Congenics program requires a maximum of five generations where candidate breeders are genotyped at each generation and those with highest percentage of the desired strain are selected to father the next generation.

Laboratory Animal Genetic Services (LAGS) provides MD Anderson investigators with cost - effective, customized genome scans using polymorphic genetic markers to support rapid congenic line development and background strain characterization for mice and rats.

Sequencing studies of cDNA from C57BL / 6J congenic mice homozygous for this allele identified nucleotide substitutions in the ORF that would cause 5 amino acid differences between the C57BL / 6J and BALB / cBy peptides, and 2 amino acid differences between the BALB / cBy and DBA / 2J peptides.

The unit provides genetic testing services using a combination of both microsatellite and single nucleotide polymorphism (SNP) panels for characterizing and selecting breeders in a wide variety of inbred mouse strains subsequently used for the generation of congenic animals.

In an operant test R6 / 2 transgenic mice (240 - CAG repeats, B6 congenic background) were less able to acquire lever pressing than control animals at an age where motor function is only mildly affected (from 9 weeks), with this deficit being both more pronounced and detected earlier (from 5 weeks) where reinforcement was not solely contingent on responding.

In particular, when made congenic on the C57BL / 6 background, retinal dysplasia is not observed.

To provide additional genetic information for congenic strains, background strain information can be ascertained using MegaMUGA, a low - cost high - density genotyping platform.

Aneuploidy was also detected in progeny from a-α opposite - sex congenic mating; thus, both homothallic and heterothallic sexual reproduction can generate phenotypic diversity de novo.

• Speed congenics Traditional backcrossing requires a minimum of 10 generations, up to three years to complete, and uses five times the number of animals.

Confirmation of quantitative trait loci influencing methamphetamine - and opioid - induced locomotor stimulation using B6.D2 congenic strains Camron Bryant, Dept of Human Genetics, University of Chicago, USA

Further, investigators frequently need to create congenic lines to study the effects of a particular allele in a different strain.

For pre-DCs transfer experiments, 1.0 × 105 sorted pre-DCs (define as Lin - CD11c + CD135 + SirpαintMHCII −) were adoptively transferred into non-irradiated congenic 3 - to 4 - week - old mice.

Chimeric mice were bred with Actin - FLPe mice (on a C57BL / 6 congenic background (N10); see Stock No. 005703) to generate the colony and remove the neo selection cassette.

The Laboratory Animal Genetic Service Core provides services for speed congenics, background characterization, microbial PCR testing, rodent STR and murine genetic consultations.

It should be noted that the phenotype of this C57BL / 6J - congenic line (Stock No. 028867 Gad2 - IRES - Cre knock - in (C57BL / 6J)-RRB- could vary from that originally described for mice on other genetic backgrounds.

The resulting C57BL / 6J - congenic strain, called Gad2 - IRES - Cre knock - in (C57BL / 6J), were sent to The Jackson Laboratory Repository in 2016 as Stock No. 028867.

As these strains are ∼ 81 % congenic, but not isogenic, they exhibit different phenotypes under various conditions, and their progeny (88 were examined) showed a range of phenotypes (Figure S11).

Of note, the same Gad2 - IRES - Cre knock - in allele is also available on a C57BL / 6N - congenic (Stock No. 019022).

Of note, Dr. Warden's lab reports that, at least once during backcrossing, a heterozygous female was bred to a C57BL / 6J inbred male (thus the Y chromosome of the congenic strain is of C57BL / 6J origin).

Identification of a congenic mouse line with obesity and body length phenotypes.

Spontaneous expansion of the CAG repeat stretch in the CAG 140 KI mouse model, which carries a chimeric mouse / human exon 1 containing around 125 CAG repeats and the human polyproline region inserted in the murine huntingtin gene (Menalled et al., 2003, Hickey et al., 2008), has recently led to a new KI line that carries around 190 CAG repeats (CAG 190 KI) in a congenic C57Bl / 6J background.

DartMouse ™ offers clients a marker - assisted congenic breeding strategy to produce congenic mice with > 99 % recipient strain genetic purity in half the time (5 generations; 1.25 - 1.5 years) instead of the more practiced 10 - 20 generations, which can take greater than 5 years.