Sentences with word «cytometric»
The word "cytometric" refers to a scientific technique that measures and analyzes the characteristics of cells, such as their size, shape, and composition, using specialized equipment called flow cytometers. Full definition
However, the results of flow cytometric analysis identified the typical CD45dim +, CD4dim +, CD56 +, HLA - DR +, and CD123 + immunophenotype.
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(A) Flow cytometric analysis of HIV - 1 gene expression in CD4 + T cells productively infected under non-polarizing, Th1, or Th2 conditions.
A flow cytometric method to measure prokaryotic records in ice cores: an example from the West Antarctic Ice Sheet Divide drilling site Journal of Glaciology, in press, p. 1 - 19.
In this series of seminars, we will discuss the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment.
Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.
Normal adult ramified microglia separated from other central nervous system macrophages by flow cytometric sorting.
Case Studies in Multi-Parameter Flow Cytometric Characterization of TCR, TIL, and CAR - T Cell Therapy Products Sadik Kassim, PhD, Mustang Bio, United States
Join us for a discussion of a broad range of novel functional reagents and demonstration of their use in cell - based immunotherapy models using powerful cytometric instrumentation.
Histology and morphology supplemented with immunohistochemistry (IHC) and / or flow cytometric immunophenotyping can be helpful to accurate diagnosis [2].
The amount and intensity of the labeled beads were determined using a Luminex xMAP cytometric scanner (BioRad).
Assisted / Supervised use The facility's staff will assist researchers in using FRACTAL strumentation, and will provide information for the preparation of samples for cytometric analysis.
The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence insitu hybridisation (FISH) experiment.
Flow cytometric analysis revealed that anti-Thy 1.2 antibody plus complement depleted > 97 % of the Thy 1.2 + cells (data not shown).
Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm — 500 nm, a relatively new flow - cytometric method — called «imaging flow cytometry» — allows for the analysis of EVs smaller than 300 nm.
In this series of webinars, we will provide investigators with the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment.
Since our flow cytometric data had shown that the HPCs poorly expressed MHC antigens [17], we wondered whether they stimulate allogenic MRL splenocytes (H2k).
When absolute purity is not necessary, as is often the case with in vitro stimulation of T cells, magnetic cell separation can deliver highly enriched cells faster, with significant cost - savings, and without exposure to harsh separation protocols like flow cytometric sorting, or chemical gradients.
Subsequent single - cell flow cytometric assays linked this shorter G1 phase to the more rapid loading of minichromosome maintenance (MCM) helicase complexes when compared to differentiated cells with longer G1 phases (neural progenitor cells and retinal pigmented epithelial cells).
(B, C) Flow cytometric analysis of HIV - 1 gene expression in (B) mock infected or (C) latently infected CD4 + T cells under non-polarizing conditions, either at the basal state or after reactivation with antibodies against CD3 and CD28.
Flow cytometric analysis of the cells following anti-CD8 antibody and complement treatment indicated that < 2 % were CD8 +.
Flow cytometric analysis was performed using BDLSR Fortessa.
Flow cytometric analysis of rabbit anti-CD3 (SP7) antibody ab16669 (1/100) in Jurkats cells (green) compare to negative control of rabbit IgG (blue).
The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.
The facility supports a range of flow cytometric techniques and currently has 9 cytometers located at F2 - 33, F2 - 34, F2 - 35 and F2 - 36.
Sensitive and viable identification of antigen - specific CD8 + T cells by a flow cytometric assay for degranulation.
The brain and meningeal material was resuspended in room temperature 60 % (vol / vol) Percoll (GE Healthcare) / RPMI and overlaid with 30 % (vol / vol) Percoll / PBS and centrifuged with no acceleration or brakes for 20 minutes at 1000 g. Mononuclear cells were harvested from the gradient interface and washed twice in PBS before preparation for flow cytometric analysis.
Flow cytometric analysis of asialoglycoprotein receptor (ASGPR) levels on the surface of hepatocytes, which were obtained from the liver specimens of patients that received hepatectomy, were used as predictors of liver dysfunction after majo...
Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).
UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.
Flow cytometric analysis (A) of MCF7, MCF7 / HER2, and BT474 cells labeled with F3 - IgG, trastuzumab, or a human IgG isotype control antibody.
(A, B) Mixed (1:1) CD11cDTR (CD45.1 +) and Ebi2 - / - or Ebi2 + / + (CD45.1 ʱCD45.2 +) BM chimeric mice were immunized with SRBCs and flow cytometric analysis of B cell responses performed five days later.
6 days after transfer, different subsets of CD45.1 + (E) or CD45.2 + (F) pre-DC derived DCs were quantified by flow cytometric analysis, with the right plots in each pair being pre-gated as shown in each left plot (n = 3 mice, one representative of two experiments).