Machine learning combined with molecular paints allows rapid screening of
changes in cell shape and function.
These changes are so fundamental that they lead to prominent
alterations in cell shape and loss of cell polarity, alterations that ultimately affect the epithelial barrier,» explained d'Azzo.
Forced expression of this SMN1 variant in cultured non-neuronal cells induces the formation of neurite - like extensions, a change
in cell shape reminiscent of that occurring when motor neurons send out axons to their muscle targets.
Methods: The team profiled lipids using analytical chemistry techniques and monitored changes
in cell shape using capabilities in the Quiet Wing at EMSL, a Department of Energy national scientific user facility located at PNNL.
In the light of a review detailing the role of these
genes in the cell shape changes leading to invagination, and of recent findings showing the expression of twist as mechanically sensitive, we suggest that the expression of twist in the mesoderm could alternatively be maintained by mechanical strains developed during mesoderm invagination.
In future, Gilmour and colleagues would like to understand the interplay between the ability — or decision — to stop and signals that they previously found drive cells to move forward, and how both are influenced by
changes in cell shape.