Sentences with phrase «labeled secondary antibody»

Subsequently, sections were stained with the appropriate fluorescently labelled secondary antibody and Hoechst dye (Invitrogen, #H3570, 1:2,000) / PBS for 1 h at RT and mounted with DAKO mounting medium.

The best way is to ensure that each of the primary antibodies is generated from a different species, in order that the fluorescent - labeled secondary antibodies used will then be detected through separate channels.

Background may derive from sticky soluble immune complexes or from contaminating bovine IgG sticking non-specifically and attracting cross-reacting labeled secondary antibodies.

Secondary labeling was carried out using isotype matched Alexa - fluor labeled secondary antibodies (AF488, 568 and 594, all from Invitrogen Corp).

Control and gesicle - treated hiPS cells were assessed for AcGFP1 expression (green), labeled with an antibody specific to pluripotency marker Oct ‑ 4, visualized with a fluorescent - labeled secondary antibody (red), and nuclear - labeled with DAPI (blue).

The antibodies are detected through a fluorescently labeled secondary antibody and a dual color system is used in order to verify the presence of the spotted proteins.

Antibody binding was visualized by chemiluminescence detection in a CCD - camera system using a peroxidase (HRP) labeled secondary antibody.

HRP RANbody staining results in a ≥ 10-fold increase in signal over staining with a primary / labeled secondary antibody and a ≥ 100-fold increase in signal over intrinsic fluorescence.

Spaghetti Monster tagged RANbodies can be detected either with a labeled anti- HA or - MYC antibody or with an anti-HA or - MYC primary and a labeled secondary antibody.

More sensitive than detecting antigen with a primary / labeled secondary antibody.

HRP - RANbodies are, however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled primary or a primary / labeled secondary antibody are used to detect additional antigens.

While Spaghetti Monster RANbody detection with a directly labeled anti-HA or - MYC antibody is less sensitive than HRP RANbody detection, it is approximately as sensitive as detecting the same antigen with a standard primary / labeled secondary antibody.

Enzyme - Linked Immunosorbent Assays (ELISAs) ELISA is an immunodetection assay that uses labeled secondary antibodies to detect specific antigens.

HuProt Cross-Section: microarray probed with a serum Ig sample and fluorescently - labeled secondary antibodies to the Ig and common epitopes of the arrayed proteins.