At least three replications (n = 3) were performed to derive the average percentage
of each cell population in each cell line and standard deviations among the replicates.
Professor Gianni Liti, a senior author on the paper from the Institute for Research on Cancer and Ageing, Nice, said: «We were able to study the evolution in time by combining genome
sequences of the cell populations and tracking the growth characteristics of the yeast cells.
Microfluidic tools for precision measurements of cell migration speed reveal that migratory speed of individual cells changes stochastically from parent cells to their descendants, while the average
speed of the cell population remains constant through successive generations.
The chambers were fixed to the stage of an inverted spinning disk confocal microscope (Nikon, Andor), which was used to capture
images of the cell populations residing on the coverslip glass.
In collaboration with experimentalists we use these methods to study self - organization
phenomena of cell populations in tissue development, disease and regeneration.
Significantly easier gating and segregation of cell populations
His research with Dr. Kerry Ressler utilizes mouse models of fear learning to better understand the molecular
identities of cell populations involved in these learning events.
«Gleevec reduces the differentiation rate of these cells so that the overall population of cancer cells decreases, but once you release the brake on the system, you have an almost explosive
expansion of the cell population because of an increase of differentiation from the stem cells that persisted during treatment,» says Michor.
PHENOMIN - CIPHE has developed a whole set of standardised protocols constituting PHENOMIN - CIPHE «toolbox», which enables to identify with unparalleled precision, the composition and the activation status of
myriads of cell populations constituting of the adaptative and innate immune system.
NSCs were generated using our previous established protocol and
consisted of a cell population with a genetic profile distinct from both human fetal cells and hESCs [36], [37].
Developing novel tools to quantify gene expression in single cells As it has become increasingly apparent that gene expression in individual cells deviates significantly from the average
behavior of cell populations, new methods that provide accurate integer counts of mRNA copy numbers in individual cells are needed.
The set of possible analyses include: 1)
comparison of cell populations for the identification of differentially expressed genes; 2) dimensionality reduction for the identification of relevant coordinates; and 3) clustering of subpopulations on the base of gene expression profiles.
The pattern of
distribution of cell populations within the colonies under these conditions of culture may be a consequence of localized paracrine and autocrine signaling.
Unlike normal plasma cells, the malignant plasma cells look large and round with a high mitotic index (measure of the
proliferation of a cell population) in early stages of cellular differentiation.