Sentences with phrase «promoter activity»
"Promoter activity" refers to the process in which a certain gene is switched on or activated within a cell. It signals the start of gene expression, allowing the cell to produce the corresponding protein or molecule. Full definition
DDX3 enhances p21waf1 / cip1 gene expression through up - regulation of promoter activity of p21waf1 / cip1.
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This observation is consistent with our in vitro studies (see Figs. 2 and 3), which indicate that DDX3 up - regulates p21waf1 / cip1 promoter activity in a p53 - independent manner.
When fused to an activation domain, ZFHD1 regulated promoter activity in vivo in a sequence - specific manner.
Both wild - type ACVR1 and mutant ACVR1 showed increased ID1 promoter activity in response to BMP treatment; however, the relative activity levels between the mutant and wild - type responses were not significantly different in these assays (data not shown).
A common FADS2 promoter polymorphism increases promoter activity and facilitates binding of transcription factor ELK1
Binding of phosphorylated Sp1 protein to tandem Sp1 binding sites regulates a2 integrin gene core promoter activity.
As shown in Fig. 4A, transient expression of DDX3 alone resulted in a 4-fold activation of p21waf1 / cip1 promoter activity whereas transiently expressed Sp1 or Sp3 of increasing amount led to a 1.6 - to 1.8-fold activation of the p21waf1 / cip1 promoter.
To examine whether DDX3 transactivates the p21waf1 / cip1 promoter activity through Sp1 site - interacting proteins, the Sp1 or Sp3 expression plasmid together with the DDX3 expression construct and the p21waf1 / cip1 promoter — driven luciferase reporter were introduced into HuH - 7 cells.
Because a similar expression level of these three DDX3 expression constructs was detected (data not shown), their differential induction of p21waf1 / cip1 promoter activity suggests that the ATPase activity, but not the RNA helicase activity, contributes to the transcriptional activation of DDX3 on the p21waf1 / cip1 promoter.
Interestingly, our data revealed that at a higher dosage (2 μg / well), the wild - type DDX3 and mutant DDX3 / AAA up - regulated the activity of p21waf1 / cip1 promoter ∼ 12-fold whereas the DDX3 / DQAD mutant only activated the p21waf1 / cip1 promoter activity ∼ 5-fold (Supplementary Fig.
A, inhibitory effect of IRF - 9 on IRF - 1 — induced RIG - G promoter activities.
A conserved core element is functionally important for maize mitochondrial promoter activity in vitro
Therefore, Sp1 plays a critical role in the regulation of p21waf1 / cip1 promoter activity.
DDX3 enhances the expression of p21waf1 / cip1 gene and up - regulates the promoter activity of p21waf1 / cip1 through an ATPase - dependent but helicase - independent mechanism.
Because the activation of p21waf1 / cip1 promoter activity was shown in various cell lines with diverse p53 status (p53 wild - type in HepG2, HCT116, and NIH 3T3; p53 mutation in HuH - 7; p53 degraded by human papillomavirus E7 in HeLa; p53 inactivated by SV40 large T antigen in 293T), this DDX3 - mediated up - regulation on p21waf1 / cip1 promoter seems to be p53 independent.
Because DDX3 up - regulates the promoter activity and the expression level of p21waf1 / cip1 gene without cell type specificity, this suggests that DDX3 acts as a general regulator of p21waf1 / cip1 gene expression.
Furthermore, DDX3 interacted and cooperated with Sp1 to up - regulate the promoter activity of p21waf1 / cip1.
Together, our results show that DDX3 exerts tumor suppressor functions, including growth inhibition and transcriptional modulation of the promoter activity of p21waf1 / cip1, and is inactivated through deregulated expression or nuclear exclusion in tumor cells.
To address the mechanism of growth inhibition by DDX3, we examined whether the promoter activity and the expression level of the cell cycle regulator p21waf1 / cip1 are regulated by DDX3.
A, DDX3 up - regulates the p21waf1 / cip1 promoter activity.
Promoter reporter analysis showed that unphosphorylated STAT6 enhanced COX - 2 promoter activities, and EMSA experiments also documented the binding of STAT6 to the COX - 2 promoter sequence.
This design permits excision of all but 238 bp of inert proviral DNA, lacking both promoter activity and polyadenylation signals, following cleavage by Cre.