Sentences with phrase «suspension culture»

To induce cardiomyocyte differentiation, compact and well - shaped EBs formed in suspension culture containing 10 % of FBS for 6 days were plated in 24 - well plates in the presence of 15 % of FBS.

We confirmed the ability of Fgf signalling to regulate the V+S + population by treating suspension cultures with the phosphatase inhibitor sodium vanadate to stimulate the FGF / Grb2 / Mek pathway.

Synchronization of Medicago sativa Cell Suspension Culture.

In this case, we began with carrot suspension cultures and have already produced small quantities of «heavy - labeled» lutein for animal trials,» Rogers said.

While the parental and hypoxia - exposed adherent cells display similar rates of proliferation, the cycling hypoxia - selected subpopulations grow slower as adherent cells and even slower as suspension cultures (Figure 4c).

(b) Neural spheres were formed in suspension culture.

«Our team has learned to grow tomato plants in suspension culture that produce lycopene molecules with a heavier molecular weight.

After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60 mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

(A) Well - shaped EBs could be formed in suspension culture (a) and maintain expression of EGFP (b).

In suspension culture, they formed embryoid bodies (EBs), which contained various cell types originating from three embryonic germ layers.

However, the industry appears to favor cells adapted to suspension cultures (e.g., HEK293), which can successfully grow in serum - free media and thus provide a heightened safety profile and reduced cost of goods (CoG)(33).

This technique exploits both the propensity of breast stem cells to generate mammospheres (3D clusters of cells) in suspension culture (21), and the relative quiescence of stem cells compared to other breast cell types, when propagated in vitro [Fig. 4].

Both normal and cancer breast stem cells give rise to 3D mammospheres in suspension culture.

Indeed, when we assessed these cells in suspension culture, overexpression of MELK significantly increased the proliferation of HMEC cells (Figure 4 — figure supplement 1A).

Indeed, we found that MELK overexpression in non-transformed HMEC - DD cells resulted in increased cell proliferation in suspension culture (Figure 4 — figue supplement 2A).