Sentences with phrase «tissue culture dishes»

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60 mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

Primary neurospheres were then collected and replated onto matrigel - coated tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

Hence, the researchers studied the growth of synapses in tissue culture dishes following exposure to a (learning) stimulus.

The cells were maintained on gelatinized tissue culture dishes.

Neuronal outgrowths in 24 - well tissue culture dishes were fixed, permeabilized and blocked as above prior to staining with anti-β-III tubulin, 1 ∶ 200 (Chemicon mab1637 followed by secondary labeling with AF488 goat anti-mouse IgG1 (A21121, Invitrogen).

Pseudomonas also will kill human lung cells in tissue culture dishes.

«You would never see these two effects if you were studying protein folding in a tissue culture dish, because you don't have the whole organism, C. elegans, in which you can look at the signals being communicated.»

We differentiated our iPS cells as embryoid bodies (EB)(Figure 4A) in the presence of FBS to induce endodermal and mesodermal fates, and in the presence of retinoic acid to induce an ectodermal fate, and after ten days allowed them to adhere to tissue culture dishes.