Sentences with phrase «transduction efficiency»
"Transduction efficiency" refers to how well a process called transduction works. Transduction is when something (like a virus or bacteria) transfers genetic material into a cell. The transduction efficiency is a measurement of how successful this transfer of genetic material is. Full definition
While many new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge.
blog.addgene.org
In addition, this study found that transduction efficiency varied not only between serum from different manufacturers but also between different lots of serum from the same source.
However, low gene therapy vector transduction efficiencies still represent a major hurdle.
Interestingly, one study found that alternative polycations such as DEAE - dextran can improve transduction efficiency.
Initial evaluation of this method showed 5 — 10 × increase in transduction efficiency.
Increases transduction efficiency and transgene expression.
At the end of the project, the transduction efficiency of the newly made retroviral vectors was tested in hematopoietic cells, and here my «old» techniques came in handy.
In general, the higher the passage number of a culture the lower the transfection and transduction efficiencies.