Sentences with word «adenoviral»

A featured paper in the February issue of the research journal Cancer Gene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differences between cancerous and normal liver cells.

Preparation and distribution of 19 reference antigens for several diseases including malaria, HIV and tuberculosis, and the establishment of five new cell banks to improve the growth of adenoviral vectors.

2/13/2007 Study Shows Liver an Excellent Target For Cancer Gene Therapy Using Viral Vectors A featured paper in the February issue of the research journal Cancer Gene Therapy demonstrates that cancer cells in the liver are excellent targets for gene therapy using adenoviral vectors, based upon a fundamental new understanding of the differen... More...

In animal models, E1 - deleted human adenoviral recombinants of the serotype 5 (AdHu5) have shown high efficacy as vaccine carriers.

Its goal is the development and characterization of novel vaccine carriers to HIV - 1 antigens based on El - deleted adenoviral recombinants derived from chimpanzee serotypes in pre-clinical animal models.

«We also found that other cancer cells, including those from the breast, pancreas and prostate, are readily infected by adenoviral vectors indicating disorganized expression of the CAR receptor in these tumor cells.

Perth biotechnology company Imugene Ltd has received notice of grant for patent protection for its Porcine Adenoviral Delivery Vector technology in the United States of America, the company has announced.

First, conventional testing for other pathogens, including other viruses by Virochip, was negative, and affected monkeys did not respond to empiric therapy with antibiotics or antivirals (ribavirin and oseltamivir in anecdotal use are not effective against adenoviral infections)[30].

Whole - genome phylogenetic analysis placed TMAdV in an independent species group separate from the known human adenoviral species A — G (Fig. 3).

Among all 95 fully - sequenced adenovirus genomes in GenBank, the closest simian adenoviral relatives to TMAdV were SAdV - 3, SAdV - 18, and SAdV - 21, with pairwise nucleotide identities ranging from 54.0 % to 56.3 % (Fig. 4).

To circumvent this problem, novel replication - defective adenoviral vaccine carriers based on El - deleted recombinants of chimpanzee - derived adenoviruses were developed.

WA - based biotechnology company Imugene Ltd has won exclusive distribution rights for its porcine adenoviral vector delivery system, after success in a hearing administered by the US patent office.

However, the high sequence divergence in the fiber protein (Table 2), as well as the absence of fiber motifs conserved among adenoviruses that bind CAR [36], [37](coxsackievirus - adenovirus receptor) or CD46 [38], [39], [40](data not shown), suggest that neither of these two human adenoviral receptors may be the attachment receptor for TMAdV.

The closest human adenoviral relatives were the species D adenoviruses, which share 54.3 % to 55.1 % identity to TMAdV, with human adenoviruses of other species slightly less similar (51.1 % — 54.6 %).

Second, the clinical presentation of pneumonia and hepatitis is consistent with the known spectrum of disease associated with adenoviral infections.

Raper, Steven et al. «Fatal Systemic Inflammatory Response Syndrome in a Ornithine Transcarbamylase Deficient Patient Following Adenoviral Gene Transfer.»

Entry vector constructs were recombined into adenoviral (pAd, Invitrogen) or lentiviral (pCSC - Zeo with Gateway recombination sites cloned into the multiple cloning site) destination vectors according to manufacturer's instructions.

For adenoviral transduction, HB1.F3 cells were incubated with purified adenovirus (1 ∶ 1000 dilution in DMEM) for 4 h and then cultured overnight in DMEM.

Our laboratory has previously used adenoviral expression as a means to transiently express therapeutic transgenes [2], [6].

VETERINARY health company Imugene has been granted a key patent in Japan for the product that is the basis of its poultry vaccines.Called the Fowl Adenoviral Delivery Vector, the product is a componen

Newly synthesized adenoviral DNA labeled by click chemistry is depicted in red, a viral DNA - binding protein in green, and the cellular DNA in blue.

Furthermore, serological evidence for cross-species adenoviral transmission events between different nonhuman primate species has been previously reported in the literature [42].

The Ad5 / F35 adenoviral vectors were generated on an E1 / E3 deleted backbone.

With the exception of SAdV - 23, these highly conserved probes are part of the core Virochip design and were derived from all available adenoviral sequences in GenBank as of 2002 [21].

Lack of preexisting virus - neutralizing antibodies in the human population suggests that these novel adenoviral recombinants may provide improved vaccine carriers for use in humans.

This research, funded by NIH, aims to test adenoviral recombinants based on simian serotypes for induction of immune responses to gag / pol / rev of HIV - 1 or SIV - 1 in a mouse model.

The gene - targeting approach developed by Suzuki and his colleagues relies on the use of so - called helper - dependent adenoviral vector to deliver large mutation - free DNA molecules into cells.

«At that time, I was treating patients with colon cancer that had spread to the liver using a very similar adenoviral vector administered in exactly the same way — direct infusion into the main artery feeding the liver,» said Reid, who is now an associate professor of clinical medicine in the UCSD School of Medicine.

So Reid and colleagues carefully re-analyzed the data from the 17 participants from the Stanford site to determine the impact of repeated adenoviral exposure on liver function, and documented that there were no significant problems.

It is also important to realize that studying a truly systemic LPL — KO mouse is difficult, as 97 % of the mice die shortly (15 days) after birth, unless they receive adenoviral rescue of LPL expression in the heart, in which case the animals have residual post-heparin LPL activity (31).

Adenovirus was produced by transfecting adenoviral expression vector into E1 - complemented cell line 293A and performing successive rounds of amplification until high titer was achieved.

The complete genome of TMAdV was found to be 36,842 base pairs in length, with a base composition of 20.8 % A, 29.8 % C, 29.8 % G, and 19.6 % T, and a GC content of 59.6 %, comparable to that of adenoviral species Groups C, D, and E in the Mastadenovirus genus.

The vaccines to be evaluated are based on an E1 - deleted simian adenoviral vector derived from a chimpanzee isolate.

Svensson, Eric et al. «Long - term erythropoietin expression in rodents and non-human primates following intramuscular injection of a replication - defective adenoviral vector.»

The research also demonstrates that in cancerous cells the receptor for adenovirus, called the coxsackie - adenoviral receptor or CAR, is expressed randomly over the surface of the cell and is exposed to the blood vessels.

Trastuzumab heavy and light chains were reformatted into a bicistronic expression cassette and cloned into adenoviral and lentiviral vectors for transient and stable expression, respectively.

We have found that adenoviral transduction results in high levels of transgene expression for at least two weeks.

In the current work we used adenoviral - type 5 (dE1 / E3)(Cytomegalovirus promoter) with human ABCA10 transgene (Ad - h - ABCA10) purchased from Vector Labs ® in order to investigate whether gene therapy can be used as a pre-treatment to enhance the efficiency of inhaled cisplatin.

Adenoviral infection is slightly toxic to HSCs, however, so Erlacher and colleagues developed an alternative approach in which purified BCL - XL could be introduced directly into isolated HSCs.

Fourth, the finding of intranuclear inclusions in diseased tissues, as well as direct visualization of adenoviral - like particles (TMAdV) in lung alveoli by electron microscopy (Figs. 1D - 2 to 1D - 4), support a primary role for TMAdV in the pathogenesis of tissue injury in affected monkeys.

Both vectors are human serotype 5, replication - defective, first generation adenoviral vectors deleted in E1a and E3 viral encoding regions.

To confirm the accuracy of the coding sequence, the sequence of each identified ORF was aligned to a database containing all adenoviral proteins in GenBank by BLASTX.

The high degree of sequence relatedness within members of each species suggests that at least some adenoviral strains may be capable of infecting both nonhuman primates and humans.

Young, healthy individuals are in general much less likely to succumb to adenoviral - related illness.

In addition, phylogenetic analyses of adenoviruses from greater apes reveal that they fall precisely into «human» adenoviral species B, C, and E [7].

In PNP's Phase I trial, an adenoviral vector is used to deliver E. coli PNP to head and neck tumors followed by intravenous administration of the prodrug fludarabine.