Sentences with phrase «expression vector»
An expression vector is a tool used in genetic engineering to transfer and express genes in cells or organisms. It acts as a vehicle to carry and deliver the desired genes, allowing them to be "expressed" or turned on to produce a specific protein or other desired product. Full definition
Clade C recombinant p17 / 24 - B protein, expressed as His - tag fusion protein in E.coli using expression vector pET24 and purified using Talon Superflow metal affinity resin.
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Clade B recombinant Nef protein, expressed as a His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.
To create viral expression vectors, a bicistronic construct was created.
To examine the effect of miR - 7 on the expression of KLF4, we ectopically expressed miR -7-1 or miR -7-2 in 231BoM and 231BrM cells by infecting corresponding lentivirus expression vector followed by qRT - PCR analysis.
The bacterial expression vector pGEX - 5 × -1 / Sp1 was constructed by introducing an EcoRI / XhoI — treated Sp1 gene fragment into EcoRI / XhoI — treated pGEX - 5 × -1 vector.
HEK293T cells were transfected with 25 nM miR - 142 precursor along with the TOPFlash or FOPFlash vector and the pCMV - Neo-APC expression vector or its mutant vector.
At ABC Biotech, I've created expression vectors and host strains using methods such as PCR, gene synthesis, cloning, codon optimization, albumin fusion, site - directed mutagenesis, and chromosomal gene integration / deletion.
Cloning in RNA expression vectors was combined with a sensitive electrophysiological assay in Xenopus oocytes in order to isolate a functional cDNA clone encoding the 5HTlc serotonin receptor.
Lentiviral expression vectors are the most effective vehicles for delivering genetic material to almost any mammalian cell
The mammalian expression vector pcDNA - SRα / FLAG - DDX 3 was constructed by insertion of a 2 - kb EcoRI / ApaI — treated fragment of the DDX3 gene derived by reverse transcription - PCR (RT - PCR) into EcoRI / ApaI — treated pcDNA - SRα / FLAG vector.
The authors first stably transduced freshly derived multipotent early passage hDPSCs with a PDGF - BB expression vector, generating cells that displayed heightened proliferation when compared to control hDPSCs.
The two PCR reactions are then mixed and transfected along with a Cas9 expression vector.
Bicistronic expression vector (pX330) expressing both Cas9 and gRNA was generously provided by Dr. Feng Zhang of Broad Institute of MIT and Harvard [6].
Selected Amrose variant fluorescent proteins were subcloned into the pCS2 + expression vector [32](EcoRI - XbaI) using the gel purified amplicons generated above.
A translational fusion of Punc - 7S in the GFP expression vector pPD95.67 (provided by A Fire) was injected at a concentration of 50 ng / μl and found to express in various head neurons but not in ventral nerve cord motor neurons.
Adenovirus was produced by transfecting adenoviral expression vector into E1 - complemented cell line 293A and performing successive rounds of amplification until high titer was achieved.
The bacterial and mammalian expression vectors for these CRISPR inhibitors are listed in the table below.
However, due to limitations in the size of constructs that can be efficiently inserted using current CRISPR / Cas technologies, complex projects including, sgRNA expression cassettes, promoters, polyadenylation, and fluorescent protein sequence can not all be incorporated into a single viral expression vector that also includes Cas9 cDNA.
May be used for the affinity purification of the SIV - Pk fusion protein from bacterial expression vectors 282 and 283.
(B) Schematic representation of the pCMV - Neo-APC expression vector that codes the full - length APC sequence together with wild - type or mutated miR - 142 targeted site located at the 3 ′ UTR.
When this clone was inserted into a mammalian expression vector and transfected into COS - 7 cells, a protein that specifically phosphorylated the agonist - occupied form of the beta 2 - adrenergic receptor and phosphorylated, much more weakly, the light - bleached form of rhodopsin was expressed.
They inserted the genetic sequence that codes for the p55 receptor into a loop of DNA called an expression vector.
CRISPR / Cas9 - mediated gene editing occurs in cells that express both expression vectors.
Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content added to the «Plasmids that Glow» and «Viral Expression Vectors» chapters, and more pictures and pro tips.
The expression vector contained the cDNAs coding for a potential fusion protein containing the GFP (EGFP), the 2A esterase of foot - and - mouth disease virus (FMDV), and HOXB4 with an additional N - terminal hemagglutinin (HA) epitope tag (eGFP2AHOXB4).
Modified genes, expression vectors, probes, modified proteins, monoclonal antibodies and receptors, cells and other chemical compounds such as small organic molecules are patentable.