In the second webinar, you will learn how to apply basic concepts of flow cytometry to topics such as cell proliferation, dead cell exclusion, and intracellular staining
techniques for transcription factors and phospho - proteins.
Besides, some other potential cis - acting
elements for transcription factors [such as CAAT / enhancer binding protein (C / EBP) and PU.1] have been also noted in the RIG - G gene promoter.
As the target
sequences for transcription factors are short, and transcription factors are tolerant of considerable variation in the sequences to which they bind, it is extremely difficult to distinguish functional binding sites in the vastness of the human genome.
By studying Arabidopsis plants for which the
genes for these transcription factors had been selectively knocked out, the group identified a single transcription factor that when inactive resulted in longer roots.
Affected family members, the group found, had inherited one or two defective copies of ZIC3, a previously unknown gene that appears to code
for a transcription factor — a protein that switches other genes on or off.
These observations indicate that the gene
for a transcription factor is located at the breakpoint of a consistently recurring chromosomal translocation in many acute leukemias and suggest a direct role for alteration of such factors in the pathogenesis of some malignancies.
Interestingly, not only the DNA that codes
for these transcription factor genes is shared among the deuterostomes, but also some of the DNA pieces that are used as binding sites for the transcription factors are conserved among these animals.
The genes
for each transcription factor were made from different bits of DNA that code for the functional parts of proteins, such as a domain that can bind to DNA and another that provides the protein with access to the cell's nucleus.
Using novel gene - array technology to measure the extent of gene expression in thousands of genes simultaneously, this study shows that as humans diverged from their ape ancestors in the last five million years, genes
for transcription factors — which control the expression of other genes — were four times as likely to have changed their own expression patterns as the genes they regulate.