Sentences with word «immunodeficient»

Moreover, the iPS cells can spontaneously differentiate or be induced into various cell types of three embryonic germ layers in vitro and in vivo when they are injected into immunodeficient mice for teratoma formation.

To improve our understanding and to build better and more translatable in vivo mouse tumor models, we are developing humanized mouse models consisting of the transplant of patient - derived tumor xenografts into immunodeficient mice reconstituted with human immune cells (isolated from the matching patient from the draining LNs or with allogenic PBMCs or LN cells).

In mouse models, carcinogen - induced tumors that arise in immunodeficient mice, when transplanted to wild - type mice, are usually eliminated by the intact immune system of the new host.

Comparison of human hematopoietic reconstitution in different strains of immunodeficient mice.

Besides, common manipulations utilized by scientists in various fields to dissect the cellular and molecular basis of selected diseases make use of highly immunodeficient strains, whose reliability depends on the stringency of the SOPF measures adopted by local housing and breeding facilities.

NSG ™ variants are the most highly immunodeficient mice and the models of choice for cancer xenograft modeling, stem cell...

Because they arise in immunodeficient hosts, they're also highly immunogenic.

Learn why the portfolio of NSG ™ models are the most versatile immunodeficient strains for oncology and drug discovery research.

When infused into mice bearing human tumors, CD26high T cells more efficiently reconstituted immunodeficient hosts and persisted long - term compared to other subsets.

In this study, the Hiroshima University researchers developed an animal model using severely immunodeficient mice whose livers were partially populated with human cells, in order to reconstruct elements of the human immune system.

To determine whether SOST impacts metastasis in animals, they modified a prostate cancer cell line to overexpress SOST, engrafted various cells lines onto immunodeficient mice, and quantified the rate of secondary tumors and of osteolytic bone lesions.

In tests on human breast cancer cells and in special immunodeficient mice with tissue grafts, the scientists found that both agents interfered with genes involved with breast cancer cell growth, resulting in more cancer cells.

Called immunodeficient IL2 receptor gamma (IL2rl) null mice, these animals lack the IL2ry receptor that humans also have as part of a functional immune system.

Such combinations should achieve elimination of all tumor cells, which is required in melanoma because single tumor cells are capable of tumor induction in immunodeficient animals.

Production of factor VIII by human liver sinusoidal endothelial cells transplanted in immunodeficient uPA mice.

This leaves immunodeficient individuals at high risk of recurrent infection, and vulnerable to conditions that would not usually be of concern to otherwise healthy individuals.

Using the highly immunodeficient NSG ™ mice [NOD.Cg - Prkdcscid Il2rgtm1Wjl / SzJ (005557)-RSB- as a host, they injected hu - PBMCs directly into the spleen along with HIV engineered to express luciferase (HIV - LucR).

Humanized mouse models, where human tumor tissue is engrafted into immunodeficient NOD SCID gamma (NSG) mice, are frequently used in oncology and infectious disease studies to provide valuable translational insights.

NOD.Cg - Rag1tm1Mom Prf1tm1Sdz / SzJ, a new immunodeficient mouse strain supporting enhanced human hematolymphoid cell engraftment.

To a lesser degree, this is similar to what happens to cats who are in some way immunodeficient.

There was scant experimental evidence for this hypothesis until 1994, when John Dick and colleagues demonstrated that leukemia - initiating stem cells (LSCs) present in the blood of leukemia patients may induce acute myelogenous leukemia (AML) when transplanted into severe combined immunodeficient mice (2).

Implementation of experimental procedures in cancerology and availability of 7 specialized laboratories of biosafety level BSL2 and BSL3, equipped with imaging (bioluminescence / fluorescence), surgery rooms with volatile anaesthesia, means of phenotypic characterization of immunodeficient mouse models, sensitization of murine tumours to radiotherapy and vectorized internal radiotherapy

These conjugates were injected i.v. into immunodeficient mice bearing human KB tumors that overexpress the folic acid receptor.

Our study - ready NSG ™ and NSG ™ - SGM3 mice are the most versatile immunodeficient strains.

However, their initial finding demonstrated that progenitor derived from iPSCs generated using lentiviral gene transduction led to the high incidence of highly aggressive tumors in immunodeficient mice after transplantation under the kidney capsule.

As these models have become more sophisticated, their application has grown and now includes a wide variety of immunodeficient strains that can be used to examine the in vivo growth of human tumors and to test new cancer treatments.

Immunohistochemical, cytogenetic and genetic analyses were performed on both the human biopsy and xenografted tumor from severe combined immunodeficient mice.

PDX models are created by implanting cancerous tissue from a human primary tumor directly into immunodeficient mouse or rat models, enabling acceleration of oncology research or drug discovery and development programs.

The research team found that injection of CAR T cells into 79 immunodeficient mice arrested the growth of GBM in 60 % of them.

Infusion of CAR T cells into an immunodeficient mouse model led to inhibition of tumor growth.

The researchers also tested a Runx2 knock - down variant of a human multiple myeloma cell line and found that it produced significantly less tumor growth in immunodeficient mice than the original human multiple myeloma cells.

Finally they showed old (immunodeficient) mice treated with cord plasma from which TIMP2 had been removed presented none of the improvements in memory performance seen using normal plasma.

Patient - derived xenographs involve the implantation of tumor tissue into an immunodeficient mouse which becomes an avatar to help identify which drug or drug combinations are most likely to be effective for an individual cancer patient.

One of the most popular models is the immunodeficient nonobese diabetic severe combined immunodeficiency (NOD scid) gamma (NSG) transgenic mouse line, which can be endowed with a humanized immune system using CD34 + hematopoietic stem cells.

Perhaps the most exciting observation occurred when fused IEC - 6 cells were transplanted into immunodeficient mice.

In subsequent tests using an immunodeficient mouse model infected by Zika, intravenous injections of sofosbuvir significantly reduced viral loads in blood serum compared to a placebo group.

When they mixed those cells with mouse follicular inductive dermal cells and grafted them onto the skin of immunodeficient mice, they produced functional human epidermis (the outermost layers of skin cells) and follicles structurally similar to human hair follicles.

Presumably, tumors induced in immunodeficient mice are highly immunogenic, and therefore easily identified and removed by a healthy immune system.

Once seeded onto a proper support, these keratinocytes appeared capable of forming a pluristratified epidermis both in vitro and in vivo as xenografts in immunodeficient mice.

In immunodeficient mice, for example, carcinogen - induced tumors develop more often and progress more rapidly than in wildtype mice.

In athymic (nude) immunodeficient mice, pinworm infections may instigate lymphoproliferative disorders that may eventually lead to lymphoma.

«They displayed all the characteristics of cancer stem cells, and less than 100 and as few as 10 cells were enough to initiate a tumor when injected into immunodeficient mice,» says Friedmann - Morvinski.

Another study in the same issue of Nature also investigated cancer immunoediting in immunodeficient mice, this time in an oncogene - driven model of sarcoma.

They developed a simple system that allows them to transplant human hepatocytes into immunodeficient mice, which can now be used to test how drugs affect the liver.

Upon orthotopic transplantation into immunodeficient mice, these organoids form normal pancreatic ducts and acinar tissue resembling fetal human pancreas without evidence of tumour formation or transformation.

They are known to be opportunistic pathogens, mostly affecting the immunocompromised or immunodeficient.

A thousand myoendothelial cells transplanted into the injured skeletal muscle of immunodeficient mice produced, on average, 89 muscle fibers, compared with 9 and 5 muscle fibers for endothelial and satellite cells, respectively.

To directly examine whether ABL kinases play a role in regulating the colonization and survival of breast cancer cells in the bone microenvironment, we injected control or ABL1 / ABL2 knockdown breast cancer cells directly into the tibia of immunodeficient mice.