Sentences with word «monoclonal»

Dr. Levitsky: My organization within Roche, Pharma Research and Early Development (pRED), has invested in antibody engineering, not only for use of monoclonal antibodies in the established ways of delivering cell - killing agents to tumors or interrupting cell signalling pathways, but also as ways to engage and manipulate the immune system's response to tumors.

The Cancer Center has a history of significant advances in cancer genetics, cancer biology, tumor immunology, and virology, including the identification of genes associated with different tumor types, the development of monoclonal antibodies used to study pathways and proteins involved in tumor development, and contributions to improved cancer treatments and diagnostic tests.

Crowe and his colleagues have developed a method for rapidly producing large quantities of human monoclonal antibodies against specific viral targets.

The difficulty in using monoclonal antibodies against tumor cells is that most of the antibodies raised will be against immunodominant antigens — all the high - visibility proteins that the immune system recognizes.

The CDI Fast - MAb ™ antibody development platform addresses the urgent need for monoclonal antibodies with unprecedented specificity to native human proteins.

A study that used stored blood samples from U.S. Air Force personnel who conducted aerial herbicide spray missions of Agent Orange during the Vietnam war found a more than 2-fold increased risk of the precursor to multiple myeloma known as monoclonal gammopathy of undetermined significance (MGUS), according to an article published online by JAMA Oncology.

Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

A neutralizing monoclonal antibody targeting the acid - sensitive region in chikungunya virus E2 protects from disease.

In humans, a comparison of bone marrow from 14 normal bone marrow donors, 35 multiple myeloma patients and 11 patients with a noncancerous condition called monoclonal gammopathy of undetermined significance (MGUS) showed that Runx2 levels were significantly higher in the multiple myeloma cells.

In multiple myeloma, normal plasma cells transform into malignant myeloma cells and produce large quantities of toxic abnormal immunoglobulin called monoclonal protein that can damage multiple organs.

Post says the study is a new take on monoclonal antibody drugs that have been used in humans.

It also focuses on therapeutic monoclonal antibodies directed specifically to disease causing proteins.

There is also an injectable medication (such as Cytopoint) that uses new monoclonal antibody therapy to target and block specific molecules that activate itching.

There is a distinct interest in developing monoclonal antibodies for use in a therapeutic setting.

Protection against rotavirus - induced gastroenteritis in newborn mice by monoclonal antibodies to surface proteins vp3 and vp7.

Arsanis is a clinical - stage biopharmaceutical company developing targeted monoclonal antibodies for pre-emptive and post-infection treatment of serious infectious diseases.

Scientists ask the same question when genetically identical cells in identical environments — monoclonal cells produced by a single ancestor that replicated — exhibit wildly different behaviors.

Membranes were then stripped, washed and re-probed with mouse monoclonal anti-human smooth muscle alpha - actin (1:10,000; Dako North America, Inc., Carpinteria, CA) diluted in 2.5 % nonfat milk.

These XT contents were subjected to culture and indirect fluorescence assay (IFA) using the anti-OspA monoclonal as above.

Depletion of CD4 + T cells by in vivo administration of anti-CD4 monoclonal antibodies (12, 13) or deletion of the CD4 gene (14, 15) have demonstrated the importance of CD4 + T cells in corneal graft rejection.

BARDA is now exploring the possibility of producing the antibodies in Chinese hamster ovary (CHO) cells — the standard system for making monoclonals.

The basis for the new understanding that there might be some universal vaccine possible is that several groups, including ours, identified and isolated monoclonal antibodies.

High levels of monoclonal immunoglobulin (myeloma protein) are present.

This research has important implications for the use of DNA - encoded monoclonal antibody technology as a platform for delivering the next generation of immunotherapies for cancer and many human diseases.

One pair of slides was incubated with the primary antibody (anti PD - L1 monoclonal mouse antibody, clone 5H1, generated by Dr. Lieping Chen, Yale University, New Haven, CT) diluted at 1:75 in ACE block at 4 °C overnight.

I made a few monoclonal lines (Figure 2) and I was surprised at how variable Cas9 - expression was across the different cells (Figure 3).

Histological sections form the lacrimal tissue were processed immunohistochemically with primary monoclonal antibodies recognizing the T lymphocyte CD3 antigen and the B lymphocyte CD79a antigen.

But they did not see the effect when they gave monoclonal dengue antibodies to live mice and then infected them with Zika.

The cells were then incubated with ab7291 at a working concentration of 0.5 μg / ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor ® 647, shown in red) at 1/250 overnight at +4 °C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor ® 488 (ab150117) at 2 μg / ml (shown in green).

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

«Engineering novel methods of delivering monoclonal antibodies could be an important approach in the fight against infection and in unique treatment situations,» said senior author David B. Weiner, PhD, a professor of Pathology and Laboratory Medicine and chair of the Gene Therapy and Vaccine Program.

Sander van Deventer has had a critical role in the development of the first commercial monoclonal antibody (Remicade) and the first gene therapy to be granted market authorization in the Western world (Glybera).

«The new potent monoclonal antibodies and the new humanized mouse models without a doubt open fantastic possibilities,» she says.

In this study, patients began treatment with chemotherapy using cyclophosphamide and topotecan and the experimental monoclonal antibody hu14.18 K322A.

Her company will satisfy a need in the marketplace for reasonably priced custom monoclonal antibodies for diagnostic and therapeutic uses, she said.

Is there any difference between the specificity of the Rabbit Anti-CD3 Monoclonal Antibody, Unconjugated, Clone SP7 cat # ab16669 and Rabbit Anti-CD3 epsilon Polyclonal Antibody, Unconjugated cat #ab49943?

Novartis currently employs over 1,200 people in Cambridge and plans to continue expanding albeit only in specific areas such as biologic drugs like monoclonal antibodies and RNA interference molecules.

«However, nanobodies are much smaller, more stable, easier to produce, and cost - effective than traditional monoclonal antibodies,» says Hansman.

Emicizumab, a novel monoclonal antibody, is given subcutaneously only once per week.

They report that the use of PD - 1 / PD - L1 monoclonal blocking antibody allows T cells to remain active and fight malignant evolution.

To investigate the intracellular Aβ pathology, we have performed high - magnification quantitative co - localization / exclusion analysis, using three different monoclonal antibodies specific for Aβ: 1) McSA1, against Aβ residues 1 — 12; 2) MOAB - 2, against Aβ residues 1 — 4 and 3) Nu1, a conformation - specific antibody which recognizes Aβ oligomers [40].