Sentences with word «mycoides»

A synthetic Mycoplasma mycoides genome transplanted into M. capricolum was able to control the host cell.

The team began the process to edit the M. mycoides genome by first cloning it in a strain of yeast expressing Cas9.

The transplanted genome booted up in its host cell, and then divided over and over to make billions of M. mycoides cells.

Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol — mediated transformation.

In March, the J. Craig Venter Institute in California unveiled Mycoplasma mycoides JCVI - syn 3.0, the first artificial species ever created.

The cell was created by stitching together the genome of a goat pathogen called Mycoplasma mycoides from smaller stretches of DNA synthesised in the lab, and inserting the genome into the empty cytoplasm of a related bacterium.

Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences.

In an attempt to complete its long - standing goal of designing a minimal genome, Venter's team designed and synthesized a 483,000 - base, 471 - gene M. mycoides chromosome from which it had removed genes responsible for the production of nutrients that could be provided externally, and other genetic «flotsam».

But the sluggish growth of natural M. genitalium cells prompted them to switch to the more prolificMycoplasma mycoides.

After several false starts, they showed that the synthetic microbe booted up and synthesized proteins normally made by M. mycoides rather than M. capricolum (Science, 21 May 2010, p. 958).

M. mycoides used in the experiments started with 901 genes.

(LA JOLLA, CA)-- August 4, 2016 — Researchers from the J. Craig Venter Institute (JCVI) and Synthetic Genomics, Inc. (SGI) have published research describing a method for engineering Mycoplasma mycoides 16S ribosomal RNA (rRNA) using a one - step process that combines CRISPR / Cas9 gene editing system with yeast recombination machinery.

The team, who published some of their first studies as early as 1999, culminated their efforts with the first synthetic cell, Mycoplasma mycoides JCVI - syn 1.0 in 2010, and in March 2016 published result of the successful construction of the first minimal synthetic cell, JCVI - syn 3.0.

Only those cells that absorbed the M. mycoides genome survived.

These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.

But this June, he and his colleagues delicately teased out the entire genome of Mycoplasma mycoides (which infects goats) and slipped it into Mycoplasma capricolum, a related but distinctly separate species.

More recently, the researchers started by stripping the M. mycoides genome down to its essentials.

In a work published in the online version of Science magazine in May 2010, whose authors were Daniel Gibson et al., they describe the synthetic assembly of the genome needed to create the bacterium Mycoplasma mycoides.

Venter's team took out the genes that allow M. mycoides to cause disease in goats.

The breakthrough, which took 15 years and consumed $ 40 million, involved building the genome of Mycoplasma mycoides (a bacterium that infects goats) from chemicals in the laboratory and then tagging it with a gene that turns the organism blue.

Venter's team, based at the J. Craig Venter Institute in Rockville, Maryland, took the genome of one bacterium, Mycoplasma mycoides, copied it and transferred it to yeast for easier modification, and then implanted it into another bacterial species, Mycoplasma capricolum.

Venter's team took the genome of one bacterium, Mycoplasma mycoides, copied and modified it in yeast, and then transplanted it into another bacterial species, M. capricolum.

A Mycoplasma mycoides large - colony genome was propagated in yeast as a centromeric plasmid, engineered via yeast genetic systems, and, after specific methylation, transplanted into M. capricolum to produce a bacterial cell with the genotype and phenotype of the altered M. mycoides large - colony genome.

A Mycoplasma mycoides genome was engineered in yeast and then transplanted into M. capricolum cells to produce a new strain.

We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell.

While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides.

Syn3.0 has a respectable doubling time of 3 hours, compared with, for instance, 1 hour for M. mycoides and 18 hours for M. genitalium.

mycoides genome into eight DNA segments and mixed and matched these to see which combinations produced viable cells; lessons learned from each cycle informed which genes were included in the next design.

In 2010, Venter's team reported that they had synthesized the sole chromosome of Mycoplasma mycoides — a bacterium with a relatively small genome — and transplanted it into a separate mycoplasma called M. capricolum, from which they had previously extracted the DNA.

The newly - created bacterium contains a minimalist version of the genome of Mycoplasma mycoides.

Last August you reported cloning the entire genome of a bacterium, Mycoplasma mycoides.

The scientists added close relative Mycoplasma capricolum (another goat pathogen) to a solution containing M. mycoides's genetic material and gently mixed it for a minute.

The researchers worked with Mycoplasma mycoides (a microbe that infects goats) because it has one of the smallest genetic blueprints of any known self - replicating organism and lacks cell walls, making it easier to insert new DNA.

We report the design, synthesis, and assembly of the 1.08 — mega — base pair Mycoplasma mycoides JCVI - syn 1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome.

Scientists at the J. Craig Venter Institute (JCVI), the International Livestock Research Institute (ILRI), and the National Institute for Agronomical Research (INRA) will join forces to use new synthetic biology technologies to create strains of Mycoplasma mycoides subspecies mycoides that can be developed as live vaccine candidates for the prevention of contagious bovine pleuropneumonia, an economically very important livestock disease within Africa.

In 2010, researchers at the J. Craig Venter Institute in La Jolla, Calif., copied the entire genome of M. mycoides.

Researchers might also start with another organism instead of M. mycoides.

The new microbe is a stripped - down version of Mycoplasma mycoides (MY - ko - PLAZ - ma My - KOY - dees).

«This new genomic platform would allow us to quickly engineer any essential gene in the «simplest» M. mycoides genome and obtain a quick, binary «yes» or «no» answer as to whether the modification introduced could support cellular viability.