Sentences with word «pluripotency»

Histone demethylation through JMJD5 is found to be vital for the cell cycle and maintenance of pluripotency in embryonic stem cells

(A) Expression of pluripotency markers from iPSCs (HiPSC - 1 control) generated following retroviral transduction of unsorted HUF1 cells.

In the current study, ZFP217 was found to regulate m6A deposition on to mRNAs created by stem cell pluripotency genes by attaching to another enzyme, m6A methyltransferase - like 3 (METTL3), and rendering it inactive.

Panel B. CD81 - negative cells were examined for pluripotency markers Oct ‑ 4, TRA ‑ 1 ‑ 60, and SSEA - 4.

Now Yamanaka and his colleagues report in the journal Cell that the same combination of genes induced pluripotency in commercially available human fibroblasts (connective tissue cells that play a crucial role in healing) derived from the facial skin of a 36 - year - old woman, the joint tissue of a man, aged 69, and a newborn, respectively.

These enhance the global translational rate, and are critical to sustain the protein levels of pluripotency factors such as Nanog and Esrrb, which can otherwise easily be disrupted.

ESCs are maintained in a state of pluripotency by a precise regulation of numerous intrinsic and extrinsic factors.

The mechanism of this two - factor reprogramming is probably largely due to the intrinsic expression of SOX2 within human NSCs, which has been shown to a major protein involved in maintaining pluripotency in hESCs.

«Like the gene itself, this region of the genome enables these stem cells to maintain their ability to become any type of cell, a property known as pluripotency.

As seen in cells dissected from colonies, significant numbers of cells coexpressed lineage specific markers along with pluripotency genes.

In order to address this question, we focus on the Nodal signaling pathway and analyze the chromatin profile of its target genes during the transition from pluripotency to fate specification.

The HIGH population expressed pluripotency genes at a greater frequency than the other cell populations, and as with the previous analysis, Oct - 4 and DNMT3b were in general more uniformly expressed than were nanog, TDGF - 1 or GDF - 3 (Figure 3a — b).

Researchers investigating signaling pathways which control pluripotency highlight newly found interactions and redundancies in mouse embryonic stem cells

We also performed qRT - PCR to determine whether the retroviral OCT3 / 4 and KLF4 transgenes were silenced, which has been shown to be a reliable measure for efficient reprogramming of somatic cells into pluripotency [29].

Previously we showed that in cultures of human ES cells grown under conditions that provide for stem cell renewal, there was a continuous gradient of expression of genes associated with pluripotency [14].

Autotaxin - mediated lipid signaling intersects with LIF and BMP signaling to promote the naive pluripotency transcription factor program.

Each of these cells, called naive, pre-implantation embryonic cells, has the capacity to develop into any cell type in the human body, an ability called pluripotency.

In accordance with previous findings in mouse ES cells, the cell populations showed heterogeneity in the expression of some markers of pluripotency at the protein level (Figure 3e — f).

Over the past year or so, various scientists have reported improvements on the technique, using fewer genes and safer viruses, or plasmids — chunks of DNA containing the necessary factors to activate pluripotency.

Chd1 may function in ESCs to maintain chromatin in an open (euchromatin) state and potentially promote pluripotency in this way.

Furthermore, data from RT - PCR analysis showed that transgenic 4 factors were completely silenced and endogenous pluripotency markers Oct4, Sox2, Rex1 as well as Nanog were successfully activated (Figure 3C).

He seeks to understand the molecular mechanisms that underlie pluripotency and the rapid proliferation of embryonic stem cells — they can become any type of cell in the body — and to identify the factors that induce reprogramming.

Numerous alternative methods for inducing pluripotency without the use of gene insertion have been reported, and although their efficiency remains problematic, if the current rate of progress in methodology continues, these should make it possible to bypass some of the primary safety concerns.

Cells do not reach pluripotency.

► The RIKEN stem cell «fiasco» continued with a 26 December report concluding that three «supposed STAP [stimulus - triggered acquisition of pluripotency] stem cell lines were actually likely to be... previously existing embryonic stem (ES) cell lines,» Dennis Normile wrote.

After analyzing the results and comparing them to the expression of a «housekeeping» gene and other pluripotency factors, Faddah concluded that, regardless of the cells» growing environment, most ES cells express both Nanog alleles and the variability of this expression corresponds to that of the other genes.

What's more, he says, the team «took advantage of the other property» of the system, which was that it could be mobilized via an enzyme, transposase, to effect the «seamless removal» of the foreign DNA without disrupting the newly gained pluripotency.

Gesicle - mediated knockout of AcGFP1 hiPS cells does not alter pluripotency when performed in the DEF ‑ CS culture system.

«Induced pluripotency seems to be the current hot topic.

New research at the Hebrew University of Jerusalem sheds light on pluripotency — the ability of embryonic stem cells to renew themselves...

The pathways that connect expression of stem cell surface glycoconjugates such as the TRA -1-60 / GCTM - 2 antigen, receptors, and growth factors in human and even mouse ES cells with the transcriptional networks that regulate pluripotency remain unclear.

These observations, which have provided striking insights into the epigenetic regulation of the molecular blueprint for pluripotency within ES cells, have been made at the population level, and thus reflect the summation of activity within all cells in the population.

It could be useful, said Garcia - Ojalvo, «to analyze protein - protein interactions in pluripotency using the modeling approach in the Science paper.»

Figuring that out could help researchers better understand what happens as cells are reprogrammed and may also provide new clues to the molecular signals that control the difference between pluripotency and totipotency.

However, is pluripotency necessary, or is unipotency enough?

Assessing pluripotency in human cells is inherently an intractable problem.

After editing, cells were analyzed for AcGFP1 expression and pluripotency via flow cytometry (Panel B) and immunocytochemistry (Panel C).

To further investigate additional similarities between hNSCs and hESCs at the transcriptional level, we analyzed the expression of key genes involved in hESC self - renewal and pluripotency utilizing semi-quantitative RT - PCR analysis.