Sentences with phrase «primary cultures»

Developed primary culture models of activity - dependent gene transcription and of HD.

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

Ten microlitres of the cell / plasmid mixture was then electroporated according to the manufacturer's instructions using the following parameters (1200 V, 20 ms, 3 pulses for SH - SY5Y cells or 1100 V, 30 ms, 2 pulses for primary cultures).

«It would seem,» he writes in his new book, «that a stable and coherent primary culture is essential for children to develop a sense of identity, which is in turn a prerequisite to developing a tolerant and loving relationship with others....

But that, as Novak points out, relies upon «the erroneous notion that democracy must create its own culture rather than drawing upon the practical wisdom of more primary cultures, cultures like Judaism that inevitably trace their wisdom back to the God who has created all and who has revealed himself to its adherents....

In Jim's lab, I learned to make primary cultures of Drosophila embryos, run Northern gels, and extract polytene chromosomes.

When primary cultures from these mice are stimulated with proinflammatory cytokines, the endothelial cell fraction responds by up - regulating the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1.

On average, the number of cells expressing both E-selectin and VCAM - 1 found in primary cultures ranged from 3 to 10 % of the total cell population.

Once primary cultures were established, the cells were immortalized with human papilloma virus E6 and E7 oncogenes using the disabled recombinant retroviral vector pLXSN16E6 / E7 (26).

A trip to Aussie would not be doing yourself justice if you did not take the time to soak up the glorious outdoor backdrop offered by this great iconic nation who's primary culture revolves around the sun, surf, sailing, tennis, hiking, water skiing and plenty more out doors activities.

Fluorescence histograms obtained from primary culture of colon cells (A) and at the time of second sorting cycle (B).

Methods: We used primary cultures of post-weaning murine mediobasal hypothalamic neurons, hypothalamic neurons derived from human induced pluripotent stem cells, and calcium imaging to characterize rapid neuronal responses to physiological changes in extracellular l - Leucine concentration.

«Dimethyl sulfoxide is a potent modulator of estrogen receptor isoforms and xenoestrogen biomarker responses in primary culture of salmon hepatocytes.»

We find ourselves with not only more than two primary cultures and traditions trying to live in harmony, but there is an extensive history of pain caused by racism in our country.

White Christians, after all, are not part of the majority culture and never have been, unless they define their primary culture as that of the United States of America.

The presence of synthetic ovine corticotropin - releasing factor leads to a rapid and marked stimulation of adenosine 3», 5» - monophosphate accumulation in an enriched population of rat pituitary corticotrophs in primary culture.

The direct application of insulin to primary cultures of the anterior pituitary did not evoke ACTH release or affect the maximal ability of corticotropin - releasing factor or epinephrine to stimulate ACTH secretion.

Primary cultures were supported at 33 °C in a mixture of 5 % carbon dioxide and 95 % oxygen, and the media was replaced as needed.

Indeed, we found that the addition of 10 units / ml IFN - γ to primary cultures and first - round sorted cells significantly reduced the time period necessary to obtain purified endothelial cell populations.

Primary cultures of cerebellar neurons from neonatal rats allow for examinations of the developmental neurotoxicity of chemicals because the various stages of neurodevelopment — including proliferation, migration, differentiation, and morphological and functional maturation — can be observed in vitro.

(a) Primary culture of NPCs of neonatal mice carrying the EGFP transgene.

Primary cultures were plated on poly - d - lysine (Sigma) coated tissue culture plates.

We do recommend that you try alternate teas in small batches (such as a one - gallon jar) apart from your main jar, so as to not risk losing your primary culture.

Reading comprehension can be more easily achieved if students are allowed to pick books with themes from their primary culture that contain references and cultural ideals they are familiar with.

Of course, if a primary culture is returned, antibiotic treatment should begin based on culture and sensitivity data.

A positive culture result obtained from a tracheobronchial wash does not necessarily imply the presence of a clinically significant airway infection and should not lead to antibiotic therapy, unless there was a pure bacterial culture on a primary culture plate.

This pedestrian only street fair is held on the Saturday of the festival weekend, and showcases art, food, and music from the five primary cultures that make up the Toledo District.

The legendary contemporary art dealer Allan Stone acquired his first African artwork in 1955, and over the next 50 years built one of the most important private collections in the world of arts from primary cultures.

· Experience in primary culture...