Sentences with phrase «tissue culture»

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

This can increase growth, perhaps because it helps the plant tolerate the stresses of tissue culture.

The researchers showed that the approach was successful both in tissue culture cells as well as in mice.

Then, by using standard tissue culture methods, they might be able to grow dinosaur tissue in the lab.

Combined with other tissue culture methods, this could reduce possible complications by producing organs that are tailored to fit the recipient's needs precisely.

The development of the brain - like tissue cultures not only opens doors to new research approaches.

The milestones along the way range from developing elephant tissue cultures to genome editing and most importantly, developing insights that help with Asian elephant conservation.

«Before, you could in principle test chemical compounds of interest against hundreds of cell lines, but it would require a lab member going to a freezer, taking out hundreds of vials — one for each cell line — and setting up hundreds of tissue culture plates.

The Van Eck lab used tissue culture techniques to multiply the plant.

Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.

For induction of differentiation, EPCs were plated on tissue culture treated plates at a high density (8 × 104 cells / cm2) for 8 days.

Cells were then plated in 24 - well tissue culture treated plates and incubated in a humidified 5 % CO2 incubator at 37 °C.

Several years ago, in tissue culture studies of mouse neurons, Dr. Shelanski found that caspace - 2 plays a critical role in the death of neurons in the presence of amyloid beta, the protein that accumulates in the neurons of people with Alzheimer's.

The latter can be primed in tissue culture by providing small numbers of dendritic cells.

For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on tissue culture treated flasks.

We combine it with the use of human tissue culture cells in which we validate some of the results obtained in egg extract.

But researchers must wait until they have more detailed data using tissue culture techniques borrowed from molecular biology before they can rule out other possibilities, such as the grasses themselves producing toxic...

Primary neurospheres were then collected and replated onto matrigel - coated tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

The probe we used in the in situ hybridizations was ribosomal RNA labeled with tritiated uridine, and we used Xenopus rRNA because it was available from tissue culture cells.

can confirm that ab16669 CD3 antibody [SP7] is sold as tissue culture supernatant.

«A new clone was «born'that, along with the new tissue culture techniques, helped save and globalise banana production.»

Barnes's team went on to show last year that soya extracts could also inhibit the growth of prostate cancer cells in laboratory tissue cultures.

Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).

«If you use conventional tissue culture techniques without this perfusion, you end up with something like a [n] M&M candy --[a joint with] a healthy surface, but dead inside,» Vunjak - Novakovic says.

Schweppe started searching the American Tissue Culture Collection (ATCC) database of nearly 1,200 fingerprinted human cell lines, both normal and malignant, from tissues, organs and tumors.

Specifically, I am investigating how the cAMP / PKA signaling pathway affects collagen structure and deposition, using a hydrogel - based, three - dimensional culture system to create a model that is more physiologically relevant than one produced by standard two - dimensional tissue culture methods.

Studies in mice and in tissue cultures suggest that giving vitamin C with tuberculosis drugs could reduce the unusually long time it takes these drugs to eradicate this pathogen.

After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60 mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

Poor tissue culture practices can lead to low titers and poor transductions.

Poor tissue culture practices can lead to

I do believe that the heart conditions in Cavalier King Charles Spaniels could be the end result of repeated immunizations by vaccines containing tissue culture contaminants that cause a progressive immune response directed at connective tissue in the heart valves.

By contrast, 67 % -100 % of control banana plants after three years were either dead or TR4 - infected, including a Giant Cavendish variant 218 generated through tissue culturing in Taiwan and reported to be tolerant to TR4

However, he hopes that the work he and Potter have carried out, which was funded by the Cancer Research Campaign, will stimulate interest in the field and make such tissue culture more readily available.

Wanting to pursue more experimental research, he headed to the Massachusetts Institute of Technology for a postdoc to join a lab of engineers who focused on cell and tissue culture experiments.

The growth medium used in plant tissue culture may be supplemented with proline.

To purify many proteins in parallel for comparative screening, a custom - built protein purification system was employed, that enables the simultaneous purification of up to 96 His - tagged proteins, even from large (> 50 mL) tissue culture volumes [33].

These bananas are sterile and dependent on propagation via cloning, either by using suckers and cuttings taken from the underground stem or through modern tissue culture.

Do neurons grown in tissue culture possess consciousness?

This sustainability work has included expanding seedling cultivation, which is done using non-GMO tissue cultures to increase availability, and increasing the crop to two a year on its trial farms.

Our standard protocol is similar to standard tissue culture requires as little as a 48h cell incubation period for RNA transfection and silencing.