Sentences with phrase «chromatin states»

I will show that we overcome major technical hurdles in accurately profiling the genome wide chromatin states of scarce in vivo cell populations by developing an highly sensitive indexing first chromatin immune precipitation approach (iChIP) which enabled us to perform genome wide ChIP with as few as 500 cells.

To study these units of life CNAG has successfully established robust sequencing - based processes to quantify gene expression of thousands of single cells and we continue developing new approaches for the identification of somatic alterations and chromatin states in single cells.

Chromatin states sometimes predicted differences in RNA expression that weren't captured by DNA methylation or accessibility measurements.

The authors first generated a common set of chromatin states across 127 epigenomes (111 of their own, and 16 more borrowed from ENCODE), all of which had been profiled for five core histone marks.

They found that keratinocytes have a different chromatin state that doesn't permit Ascl1 to bind, so what they thought or what they believe is that a keratinocyte may not be permissive to neuron reprogramming.

Can understanding the molecular mechanisms that govern chromatin state and enhancer plasticity during cellular stress provide new insights into the pathogenesis of cardiovascular and metabolic diseases?

In any given cell, certain chromatin states tend to be marked with specific histone modifications:

Luke Buchanan (Stewart, TUD)-- «Mechanisms of chromatin state definition in Schizosaccharomyces pombe» (2008)

Thus, such mutations might alter the protein function on chromatin state regulation, sensitizing PTCL - NOS patients to HDAC inhibitors.

McGarvey, K. M.; Fahrner, J. A.; Greene, E.; Martens, J.; Jenuwein, T.; Baylin, S. B. Silenced tumor suppressor genes reactivated by DNA demethylation do not return to a fully euchromatic chromatin state.

To be also able to characterize the single cell genome and epigenome, we are developing new approaches for the identification of somatic alterations or open chromatin states.

These services are aimed to deliver high quality sequencing - ready libraries to map chromatin states (histone modifications), or profile binding of epigenetic modifiers (transcription co-regulators) or DNA binding proteins (transcription factors) on a genomic scale.

Researchers at the University of Tokyo have identified modes of Sox9 action during cartilage formation by analyzing big data on Sox9 location, chromatin state, and gene expression over the whole mouse genome.

By studying the chromatin state of zygotes, we aim to gain insights into this mechanism, which could also have applications for regenerative medicine,» says Tachibana - Konwalski, underlining her excitement for the potential applications for her favourite research topic.

«Ideally, we would change the chromatin state before we start overexpressing NEUROG1 and prevent unwanted stem cell proliferation,» he said.

Next, the authors turned to their other epigenomic profiling datasets — DNA accessibility (DNAse - Seq), methlation (bisulfite sequencing), and RNA transcription (RNA - Seq) to examine and compare the properties of these chromatin states.

Using the five core histone methylation marks I've just described, the authors trained a chromatin state model that classified every region into one of 15 states:

Opening the way to accurately profile the chromatin states of in vivo stem cells, lineage progenitors and other scarce cell populations.

According to this model, differentiation is a gradual transition from an open and permissive chromatin state in multipotent stem cells to a compacted cell - type specific chromatin state in differentiated cells.

In this new research line, we initially will study how transcription factors, RNA polymerases, and other molecules are partitioned in the nucleoplasm, and how they contribute to the establishment of structures such as transcription factories, chromosome territories or chromatin states.

However, researchers from the laboratories of Ralph Stadhouders, Marc A. Marti - Renom, and Thomas Graf have now applied a highly efficient and synchronous reprogramming system [2, 3] to study how genome topology, chromatin states, and gene expression dynamically change during reprogramming [4].

Overall, this study highlights the close links between transcription factor - driven genome topology dynamics, chromatin state, and gene expression and highlights a critical role for genome topology in enforcing transcriptional programs and cell fate.

efficient and synchronous reprogramming system, researchers describe the dynamic alterations to genome topology, chromatin states, and gene expression

Cell - fate conversion requires the dynamic reorganization of chromatin states and alterations to the active and repressed sub-nuclear compartments

Using an efficient and synchronous reprogramming system, researchers describe the dynamic alterations to genome topology, chromatin states, and gene expression