Sentences with word «epitope»

Some targeted epitopes in the second variable (V2) loop of HIV Envelope, while others targeted glycans.

This approach is considered «passive,» as nanoparticles do not use ligands targeting specific epitopes on the cell surfaces.

I am interested in understanding how dengue virus and zika virus - specific T - cell responses can mediate protective immunity or disease pathogenesis, and in defining new protective T - cell epitopes for immunotherapeutic reagents or novel vaccine development.

Removing the cytoplasmic domain may distract the immune response and instead generate antibodies that target epitopes on Env that would not lead to protection.

Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»

The haptens were then attached to carrier proteins called epitopes that provide a binding site for antibodies.

«As eliciting a highly diverse immune response may be favorable to providing protection against incredibly diverse HIV - 1 variants in global circulation,» the researchers conclude that their study «supports further investigations of the molecular and functional characteristics of the virus - antibody interplay in superinfected individuals, as superinfection may provide insight to the development of a diverse Nab response with multiple epitope specificities.»

Another company, EpiVax Oncology, bases its neoantigen approach on mutated tumor - specific epitopes, called T cell neoepitopes, which are recognized by the immune system as «non-self.»

The reactivity of this monoclonal antibody was not dependent on the V (L) or C (L) isotype of the fibril, but rather seemed to be directed toward a beta - pleated sheet conformational epitope expressed by AL and other amyloid proteins.

Cells were then analyzed by flow cytometry after being stained simultaneously with anti-CXCR4 clone 4G10, which recognizes the N - terminus, and clone 12G5 whose epitope includes the second extracellular loop that is disrupted by the X4 - ZFNs.

The antibodies that developed in these volunteers targeted different epitopes on the virus.

Furthermore, for the first time, we apply immunogold to identify protein epitopes at high resolution, by localizing antibody - antigen complexes to specific fossil ultrastructures.

Finally, Dr. Sette has overseen the design and curation efforts of the national Immune Epitope Database (IEDB), a freely available, widely used bioinformatics resource, since its inception in the early 2000s.

He is working on the prediction and validation of conserved T cell epitopes from prevalent swine influenza viruses.

The algorithm was able to predict with up to 90 percent accuracy the flu epitopes recognized by these cells.

They do this through a molecular «identity parade», which enables them to recognise epitopes — small peptide «knobs» on foreign proteins.

I have been working on the Immune Epitope Database and Analysis Project (IEDB) since 2005.

However, as the spliced epitopes are mixed sequences, they also have the potential to overlap with the sequences of healthy signallers and be misidentified as harmful.

ß1 - Adrenergic Receptor Contains Multiple IAk and IEk Binding Epitopes That Induce T Cell Responses with Varying Degrees of Autoimmune Myocarditis in A / J Mice.

In contrast, the tier 1 NAbs targeted linear epitopes in the gp120 V3 region.

Unlike antibodies elicited by annual influenza vaccinations, most neutralizing antibodies induced by pandemic H1N1 infection were broadly cross-reactive against epitopes in the hemagglutinin (HA) stalk and head domain of multiple influenza strains.

We predicted that these diversified epitope regions would be the targets of mutation as the 2009 H1N1 pandemic (pH1N1) lineage evolves in response to the development of population - level protective immunity in humans.

A viral epitope inserted into the complementarity - determining region 3 (CDR3) loop of the heavy chain of a self immunoglobulin (Ig) molecule was generated from the Ig context and was presented by I - Ed class II molecules to virus - specific, CD4 + T cells.

Based on these observations, we postulate that the plasmablasts producing these broadly neutralizing antibodies were predominantly derived from activated memory B cells specific for epitopes conserved in several influenza strains.

These proteolytically cleaved and stabilized trimers, designated BG505 and B41 SOSIP.664 gp140, display multiple bNAb epitopes and have antigenic properties that mimic viral Env.

The Sette lab's approach uses epitopes as specific probes to define the immune signatures associated with productive / protective immunity versus deficient immunity / immunopathology.

CD8 T cells specific for the noninflationary M45 epitope displayed a classic central memory phenotype, re-expressing the lymph node homing receptor CD62L and homeostatic cytokine receptors for IL - 7 and IL - 15, and produced low levels of IL - 2.

Using sera from malaria - immune adults, it has been shown that the expressed recombinant proteins contained heat - labile epitopes suggesting that they adopt their native conformation and are likely to be biochemically active.

We first examined differences between B6 Prf1 − / − HLH responses in naive mice and mice immunized against the CD8 LCMV epitopes gp33 and NP396 using a well - characterized DC immunization protocol (15).

TCRdist allowed the scientists to identify T cell receptors that recognized the same epitope.

We have turned the problem on its head and only use previously - tested epitopes.

Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule

Grouping T cell receptors for a given epitope revealed underlying common features that characterized the bulk of the repertoire,» said first author Pradyot Dash, D.V.M., Ph.D., a staff scientist in Thomas» laboratory.

Our collaboration has found a way to select epitopes reaching full population coverage.

The most potent neutralizing antibodies were ZIKV - specific and targeted EDIII or quaternary epitopes on infectious virus.

CXCR4 cell surface and intracellular expression was detected by flow cytometry after co-staining with the N - terminal specific CXCR4 antibody 4G10 and the extracellular loop (ECL) specific antibody 12G5 whose epitope includes the CXCR4Δ18 deleted residues [42].

The design, structures and therapeutic potential of protein epitope mimetics.

Genomes for many of these pathogens are becoming available due to research efforts worldwide, and so the iCubed is collaborating with researchers to identify new epitopes and design vaccines using the CCHI funded «iVAX» toolkit.