Sentences with word «kilobase»

The authors of this study have developed a universal primer set across flowering plants that amplifies 3 - 15 kilobase fragments, which can then easily be sequenced using recently developed next - generation sequencing technologies.

Bellen claims the new methodology will work for DNA segments up to 150 kilobases in length — the fruit fly genome is made up of an estimated 14,000 genes, only 10 of which, according to Bellen, are too large to be manipulated with P [acman].

However, we here show that Pseudomonas koreensis P19E3 harbors multiple, near identical repeat pairs up to 70 kilobase pairs in length.

Although mature miRNAs are only ~ 22 nucleotides, their transcripts are up to hundreds of kilobases long.

Negative frequency dependent selection acting at the locus responsible of self - incompatibility in Arabidopsis represents one of the strongest cases of balancing selection, and yet we have shown that its indirect effects on neighboring regions are restricted to a few kilobases [2].

CML cells have two novel products: an 8.5 - kilobase RNA transcript containing both abl and bcr and a 210 - kilodalton phosphoprotein (P210) recognized by v - abl - specific antisera.

By using these reagents and v - abl - specific antisera, it was demonstrated that the P210 in CML cells is indeed the protein product of the 8.5 - kilobase transcript.

The assay targets the 3.4 - kilobase mitochondrial DNA deletion and is designed for use on real - time PCR platforms.

This analysis revealed that a SNP called rs9349379, located hundreds of kilobases away, was the strongest risk factor for cardiovascular disease at chromosome 6p24.

The 3.5 - kilobase messenger RNA was detected in mammalian brains and human thymus.

RNA blot analysis revealed a messenger RNA of four kilobases with highest amounts in brain and spleen.

To test whether the P210 is the product of the novel 8.5 - kilobase bcr / abl fusion transcript, antibodies were prepared against c - abl and bcr determinants.

To determine whether the neuronal form of pp60c - src is encoded by a brain - specific messenger RNA, a mouse brain complementary DNA (cDNA) library was screened with a chicken c - src probe and a 3.8 - kilobase c - src cDNA clone was isolated.

One of the noncoding de novo mutations was 254 kilobases downstream of FGFR2, in a noncoding region that looks (according to chromatin marks) like a neural crest enhancer.

In truth, human chromosomes are tightly organized and packed into the nucleus, a situation which causes physical interaction between regions that might be hundreds of kilobases apart.

The resulting paired - end reads (75 - mers) have a «read2» that's the same for all pieces of the same kilobase - fragment, but a read1 that comes from some random location within the fragment.

Atsushi Nakabachi, a postdoctoral research associate in UA's department of ecology and evolutionary biology and a visiting scientist at RIKEN in Wako, Japan, is the first author on the research report, «The 160 - kilobase genome of the bacterial endosymbiont Carsonella.»

The complete 1.66 - megabase pair genome sequence of an autotrophic archaeon, Methanococcus jannaschii, and its 58 - and 16 - kilobase pair extrachromosomal elements have been determined by whole - genome random sequencing.

This set of proteins can process seven of the eight base - base mismatches in a strand - specific reaction that is directed by the state of methylation of a single d (GATC) sequence located 1 kilobase from the mispair.

First, they sheared DNA into fragments that were a few kilobases long, ligated adapters to the ends of each fragment, and did a round of amplification.

A positional cloning approach was used to fine - scale map the ws1 locus to a 13.5 kilobase region.

Thus, Shendure and his group sought to combine some Tech D cleverness with Illumina's incredible read depth to generate localized assemblies of kilobase - length fragments.

Overlapping «cassettes» of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb («1/8 genome»), and 144 kb («1/4 genome»), which were all cloned as bacterial artificial chromosomes in Escherichia coli.

Long - range PCR is a method that allows for the amplification of much larger fragments of DNA than is possible with traditional PCR — fragments larger than 40 kilobases have been reported in long PCR, versus fewer than 10 kilobases for traditional PCR.

The human UFD1L gene was deleted in all 182 patients studied with 22q11 deletion, and a smaller deletion of approximately 20 kilobases that removed exons 1 to 3 ofUFD1L was found in one individual with features typical of 22q11 deletion syndrome.

«Searching 1.5 kilobases of DNA is quite a distance, and 2 - 3 seconds is a long time in molecular terms to remain associated with the DNA.»

Everything hybridized until I was left with just a 38 - kilobase protein and a 24 - kilobase protein representing viral DNA.

In addition, researchers have trouble inserting and manipulating large pieces of DNA (bigger than 30 kilobases), because of the bacteria used as a vector for this DNA.

We also constructed phylogenetic trees from 14 kilobases of nuclear genes for representatives from most major primate lineages, both extant colugos, and multiple treeshrews, including the pentail treeshrew, Ptilocercus lowii, the only living member of the family Ptilocercidae.

«First we had to develop a custom scaffold strand that contained 51 kilobases.

RNA - Seq data is reported as average RPKM (reads per kilobase per million mapped reads), generated by the Genotype - Tissue Expression (GTEx) project.

When the team combined the technology with other methods, they ended up with 10,000 pieces of DNA, each longer than 10 kilobase pairs — longer than 10,000 pairs of the biological compounds that make up DNA.

As the authors put it, these analyses «portray a complex landscape of long - range gene - element connectivity across ranges of hundreds of kilobases to several megabases, including interactions among unrelated genes.»

The human T cell receptor beta (TRB, previously known as TCRB) gene locus on chromosome 7 (7q34) includes 65 Vb (variable) gene segments, followed by two separate clusters of genes each containing a Db (diversity) gene, several Jb (joining) genes, and a Cb (constant) region spread over 685 kilobases.

The density of variants (an informal proxy for purifying selection) differed as well: 6.7 and 6.5 variants per kilobase in DHS peaks and footprints, respectively, compared to 4.2 variants per kilobase in the exome.

Then, it's possible to perform a localized assembly for each kilobase fragment.

Unfortunately, LOH regions tend to be kilobases or megabases in size, containing dozens or hundreds of genes, and identifying which ones are truly affected in terms of cancer remains challenging.

To compare the expression values across genes and conditions, raw count data was transformed into fragments per kilobase of exon per million fragments (FPKM) with the formula: