Sentences with word «lentiviral»

The study also examines the use of lentiviral vectors for cell delivery in treating liver diseases, a tool traditionally used in treating blood disorders.

The library consists of a pool of lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells» genomes upon infection.

Kim Y - S, Wielgosz M, Hargrove P, Kepes S, Gray J, Persons D, Nienhuis A. Transduction of Human Primitive Repopulating Hematopoietic Cells with Lentiviral Vectors Pseudotyped with Various Envelope Proteins.

Mice generated using lentiviral vectors with muscle - specific and T lymphocyte — specific promoters expressed high levels of GFP only in the appropriate cell types.

Expression of human immunoglobulin within adenovirally - transduced HB1.F3 NSCs (HB1.F3.Ad - H2IgG; Fig. 1C) and HB1.F3.Lenti - H2IgG, a stable cell line generated by lentiviral transduction of HB1.F3 NSCs (Fig. 1D), was visualized using immunocytochemistry.

The study, titled «Restoration of spermatogenesis by lentiviral gene transfer: Offspring from infertile mice,» was funded by the National Institutes of Health, the March of Dimes Birth Defects Foundation, the Wayne and Gladys Valley Foundation, and the H. N. and Frances C. Berger Foundation.

Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments.

However, their initial finding demonstrated that progenitor derived from iPSCs generated using lentiviral gene transduction led to the high incidence of highly aggressive tumors in immunodeficient mice after transplantation under the kidney capsule.

After co-transfection of these plasmids, 293T cells released transgene - containing lentiviral particles into the media, which could be collected for experimental use.

pSIN - KLF4 - puro lentiviral plasmid was constructed by subcloning from pLM - mCherry - 2A - KLF4 purchased from Addgene.

Is this abortive infection and pyroptotic cell death not operating in non-pathogenic lentiviral infections of natural hosts?

The blog provides tips for working with viruses including advice on how to titer lentiviral preps, troubleshoot viral transductions, and overviews for beginners just starting work with virus.

A self - inactivating lentiviral construct containing a mouse phosphoglycerate kinase - 1 internal promoter driving the human gene encoding GDNF [44] was used to generate GDNF - secreting hNPCctx.

Lentiviral shRNA — mediated knockdown of ABL1 / ABL2 and expression of mouse Abl1 / Abl2 were conducted as described previously (20, 21, 59).

Many of Addgene's most requested plasmids are used with lentiviral expression systems.

Prior to infection, high - titer lentiviral stocks were obtained by ultracentrifugation and the particle content of individual batches was determined by p24 antigen ELISA and RT - qPCR quantification of viral RNA [45].

The developed vector (1TAX) combined a triple combination of anti ‐ HIV genes (TRIM5, CCR5 shRNA, and a TAR decoy) and a truncated / mutated form of human CD25 (tCD25) as the cell surface selectable marker, in a self ‐ inactivating (SIN) third ‐ generation lentiviral vector.

Review of «Safety and efficacy of a tCD25 pre-selective combination anti-HIV lentiviral vector in human hematopoietic stem and progenitor cells» from Stem Cells by Stuart P. Atkinson

High level erythroid lineage - directed gene expression using globin gene regulatory elements following lentiviral vector mediated gene transfer into primitive human and murine hematopoietic cells.

The effect of freeze - thaw cycles on lentiviral stability depends on a variety of factors such as the transgene expressed, the viral storage buffers used, and other experimental parameters.

She designed lentiviral vectors capable of inducing dendritic cell (DC) precursors into personalized cell vaccines tailored for immune regeneration.

Dr. Levine and the CVPF produced cells for the first infusions of purified activated CD4 + T cells as adoptive immunotherapy, directed the first lentiviral transduced cell infusions, and the first infusions of zinc - finger nuclease - modified CD4 T cells.

OMC also aims at potentiating the existing Conditional Mutagenesis Facility by implementing novel approaches to attain tissue -, stage - and site - specific transgene expression in mouse mutant strains, mainly through lentiviral vector - mediated transduction of blastocysts.

A successful lentiviral infection begins with your cells, both your packaging cells and your target cells.

A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules.

Since lentiviral vectors integrate into the genome, they could promote oncogenesis by altering local gene expression.

Lentiviral barcoding libraries have been used for complex applications like

A Pilot Feasibility Study of Gene Transfer For X-Linked Severe Combined Immunodeficiency (SCID) in Newly Diagnosed Infants Using a Self - Inactivating Lentiviral Vector To Transduce Autologous CD34 + Hematopoietic Cells

Basic retroviral and lentiviral procedures have been previously described (32, 33).

The variety of new tools available for genetic manipulation now include lentiviral - based gene delivery, and gene editing using CRISPR / Cas9, zinc finger nucleases (ZFNs) or transcription activator - like effector nucleases (TALENs).

Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.

The plasmids expressing hsa - mir -7-1 and hsa - mir -7-2 precursors in lentiviral pCDH - CMV - MCS - EF1 - copGFP vector (System Bioscience) were supplied by Yin - Yuan Mo (University of Mississippi Medical Center).

This research has thus far led to the discovery of multiple lentiviral restriction blocks in HSC and the development of novel, more efficient transduction protocols.

Heritable and inducible gene knockdown in astrocytes or neurons in vivo by a combined lentiviral and RNAi approach.

Many times lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping.

Consequently, it is best to avoid freeze - thawing lentiviral preps unless absolutely necessary.

Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting in lower lentiviral titers.

Tracking differentiating neural progenitors in pluripotent cultures using microRNA - regulated lentiviral vectors

Researchers are also working to make lentiviral vectors safer.

The results are the first involving infants with XSCID treated with the St. Jude lentiviral vector.