Sentences with phrase «lentiviral vectors»
Lentiviral vectors are tiny vehicles that can carry and deliver genetic material into cells. They are used in medical and scientific research to help introduce new or modified genes into cells for various purposes, such as studying gene functions, treating genetic diseases, or creating new therapies. Full definition
The study also examines the use of lentiviral vectors for cell delivery in treating liver diseases, a tool traditionally used in treating blood disorders.
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Kim Y - S, Wielgosz M, Hargrove P, Kepes S, Gray J, Persons D, Nienhuis A. Transduction of Human Primitive Repopulating Hematopoietic Cells with Lentiviral Vectors Pseudotyped with Various Envelope Proteins.
Mice generated using lentiviral vectors with muscle - specific and T lymphocyte — specific promoters expressed high levels of GFP only in the appropriate cell types.
He has pioneered the development and applications of lentiviral vectors for gene transfer, which have become one of the most widely used tool in biomedical research and, upon recently entering clinical testing, are providing a long sought hope of cure for several currently untreatable and otherwise deadly human diseases.
Preintegration HIV - 1 inhibition by a combination lentiviral vector containing a chimeric TRIM5 alpha protein, a CCR5 shRNA, and a TAR decoy.
Germline Transmission and Tissue - Specific Expression of Transgenes Delivered by Lentiviral Vectors
Cronin J, et al. «Altering the tropism of lentiviral vectors through pseudotyping.»
Review of «Safety and efficacy of a tCD25 pre-selective combination anti-HIV lentiviral vector in human hematopoietic stem and progenitor cells» from Stem Cells by Stuart P. Atkinson
High level erythroid lineage - directed gene expression using globin gene regulatory elements following lentiviral vector mediated gene transfer into primitive human and murine hematopoietic cells.
Since lentiviral vectors integrate into the genome, they could promote oncogenesis by altering local gene expression.
The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and transfer plasmids.
Therapeutic levels of fetal hemoglobin in erythroid progeny of -LCB- beta -RCB-- thalassemic CD34 + cells following lentiviral vector - mediated gene transfer.
Please see our previous post «Your Lentiviral Plasmid FAQs Answered» or our Lentiviral FAQ webpage for more detailed information on lentiviral vectors including Biosafety FAQs.
Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.
She designed lentiviral vectors capable of inducing dendritic cell (DC) precursors into personalized cell vaccines tailored for immune regeneration.
In vivo administration of lentiviral vectors triggers a type I interferon response that restricts hepatocyte gene transfer and promotes vector clearance.
Many times lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping.
«The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events.»
Tracking differentiating neural progenitors in pluripotent cultures using microRNA - regulated lentiviral vectors
Researchers are also working to make lentiviral vectors safer.
VCN and RCL Testing for Lentiviral Vectors Lindsay Skrdlant, PhD, Stanford University School of Medicine, United States
The results are the first involving infants with XSCID treated with the St. Jude lentiviral vector.
The Gibco ™ LV - MAX ™ Lentiviral Production System is the first system that provides a scalable and high - yield lentiviral vector production on a serum - free suspension platform, which facilitates the research from bench to clinic.
Efficient gene transfer into rhesus repopulating hematopoietic stem cells using a simian immunodeficiency virus - based lentiviral vector system.
Sustained high - level polyclonal hematopoietic marking and transgene expression 4 years after autologous transplantation of rhesus macaques with SIV lentiviral vector - transduced CD34 + cells.
Lentiviral vector technology has come a long way since the 1990s, both in terms of research impact and safety.
Extended ß - globin locus control region elements promote consistent therapeutic expression of a γ - globin lentiviral vector in murine ß - thalassemia.
In these particular experiments, we generated A549 cells stably expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9 - Blast (52962 - LV, Figure 1).
Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein used to psuedotype most lentiviral vectors.
A Pilot Feasibility Study of Gene Transfer For X-Linked Severe Combined Immunodeficiency (SCID) in Newly Diagnosed Infants Using a Self - Inactivating Lentiviral Vector To Transduce Autologous CD34 + Hematopoietic Cells
Briefly, 293FT packaging cells were transfected with pLL3.7 - GFP lentiviral vector, together with the packaging plasmids, using Fugene 6.
Lentivirus was produced by cotransfecting replication incompetent lentiviral vector with psPAX2 (Addgene, 12260) and pLP / VSV - G (Invitrogen).
lentiviral vectors contain a deletion in the 3» LTR that prevents aberrant activation of nearby genes.
These images show the development of cancer (dark purple) in the mouse lung, initiated by lentiviral vector.
Cronin, J., Zhang, X.Y., Reiser, J. «Altering the tropism of lentiviral vectors through pseudotyping.»
Safety and Efficacy of a tCD25 Preselective Combination Anti-HIV Lentiviral Vector in Human Hematopoietic Stem and Progenitor Cells.
This is the first published report of lentiviral vector mediated TCR therapy that has shown persistence beyond one month, June said.
Integrase - defective lentiviral vectors: progress and applications.
Since lentiviral vectors can deliver a large amount of DNA (~ 8 kb) with a relatively low immune response, it should come as no surprise that researchers are interested in developing these vectors for gene therapy.