Sentences with word «midgut»
«Biochemical networks mapped in midgut of Aedes aegypti mosquitoes.»
sciencedaily.com
In addition, cultures of tick midgut contents were pelleted and stained for IFA (G, H).
Set 2 is tissues from mice inoculated with midgut contents of ticks fed upon monkeys and those mice were then also subjected to xenodiagnosis (xeno / scid / xeno).
No guts, no glory: Biologists receive nearly $ 2.8 million from NIH to study virus escape from mosquito midgut
Set 1 is from mice inoculated with midgut contents of ticks fed upon monkeys (xeno / scid).
Initially, we stained the XT midgut smear using DFA with polyclonal anti-Borrelia species antibodies.
Panel A = positive control for IFA using B. burgdorferi culture; Panel B = XT from animal IH11 (treated); Panel C = XT from animal IK14 (treated); Panel D = XT from animal IL09 (treated); Panel E = positive control for DFA using midgut smear of tick that was capillary tube - fed B. burgdorferi; Panel F = XT from animal IP55 (untreated); Panel G = XT culture pellet from animal IP55 (untreated); Panel H = XT culture pellet from animal IK14 (treated).
W. R. Harvey, S. Nedergaard, Sodium - independent active transport of potassium in the isolated midgut of the Cecropia silkworm.
To probe alternative modes of ISC replacement, Ohlstein and colleagues developed a physiologically - relevant starvation assay that elicits a rapid and severe loss of ISCs in the Drosophila posterior midgut.
Finally, stomodeal - specific RNAi - mediated silencing of Twist during compression impairs the differentiation of midgut cells, resulting in larval lethality.
These experiments show that mechanically induced Twist upregulation in stomodeal cells is necessary for subsequent midgut differentiation.
Mario Sobern of the National Autonomous University of Mexico and his colleagues investigated the most commonly used Bt toxins, which work by binding to specific receptors in pest midguts.
Like the human intestinal tract, the mosquito midgut contains a diverse microbial flora, which may compromise the ability of Plasmodium to establish infection.
Insect midgut - dwelling bacteria generate reactive oxygen species that inhibit malaria parasite development.
In an organ (blue, bottom) ringing the fly midgut, Wigglesworthia bacteria churn out B vitamins, including B1.
It was thought that the sugar molecules were removed from lyciumoside IV in the Manduca sexta midgut and the released toxin caused these deleterious effects.
Hours later the glow showed up in the developing midgut.
Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome — Laura B. Dickson — BMC Parasites & Vectors
The mother's midgut begins to break down while she is still guarding her eggs.
A systems level analysis reveals transcriptomic and proteomic complexity in Ixodes ricinus midgut and salivary glands during early attachment and feeding.
Tissue - and time - dependent transcription in Ixodes ricinus salivary glands and midguts when blood feeding on the vertebrate host.
Many genes were expressed in previously unappreciated regional patterns, suggesting they are relevant to diverse aspects of midgut function.
The GFP expression patterns driven by the most useful lines in 5 — 10 day old adult female midguts (Figure 1 — figure supplement 2) are summarized in Figure 1D — E.
Credit for the discovery of detailed midgut regionalization deserves to be shared equally between the two groups.
When a flea feeds, blood flows into its sac - like midgut.
The tick midgut contents were also subjected to RT - PCR.
Standard PCR and RT - PCR for ospA, ospC and flaB were also performed with either SCID mouse tissues or XT midguts using primers described elsewhere [35].
First, the lining of mosquito midgut (equivalent of stomach and small intestine) has properties that can prevent the pathogen or parasite from getting out of the gut.
Tick midguts were stained for IFA with an anti-OspA monoclonal antibody, followed by an Alexa - IgG - 488 (green)- conjugated secondary antibody (B, C and D) or stained for DFA using an anti-Borrelia sp..
For example, intestinal stem cells (ISCs) of the adult Drosophila midgut (Ohlstein and Spradling, 2006; Micchelli and Perrimon, 2006) generate the two major differentiated cell types of the gut, enterocytes and enteroendocrine cells, throughout life.
A portion of the XT midgut contents were also placed in culture.
The remaining ticks were subsequently fed to repletion on mice, and midguts from a subset of ticks were examined by IFA.
When food exits the pouch, it shoots straight into her midgut, continuing largely undigested until it reaches the Y junction and combines with the acidic brew of stomach juices.
Fertilization between male and female parasites in the mosquito midgut (see paragraph on the malaria cycle) did occur, but shortly afterwards, this new generation died without infecting the mosquito.»
Fertilization takes place in the mosquito's midgut.
CSU researchers had expected to find that one virus would prove to be dominant and outcompete the others in the midgut of the mosquito where the infections establish and replicate before being transmitted to humans.
Malaria parasite transmission depends on the successful transition of Plasmodium through discrete developmental stages in the lumen of the mosquito midgut.