Sentences with word «agarose»

The «prize,» placed at the exit of each maze, was a lump of agarose gel soaked in hydrochloric acid.

The resulting DNA fragments are analyzed by agarose gel electrophoresis.

The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.

The design differs from that of a conventional commercial microscope: Keller placed the laser and objective lens at right angles to each other atop a table; then he embedded zebrafish embryos in agarose in a sample tube between the two.

Clade B recombinant Nef protein, expressed as a His - tag fusion protein in E. coli using expression vector pET24 and purified using Ni - agarose columns.

Samples were pre-cleared with protein A agarose slurry containing 10 mg / ml of E. Coli tRNA for 30 min at 4 °C.

This is 1 million times more sensitive than the typical concentration for an analysis on agarose gel.

Immune complexes were than isolated with E.coli tRNA / Protein A agarose beads, and the obtained purified DNA with subjected to qPCR using primers for HMOX1 E2 promoter.

Immune complexes incubated with protein A agarose slurry containing tRNA for 1 hr at 4 °C with rotation.

This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.

Runtimes are approximately one hour versus up to 16 hours needed for methods such as agarose - based, pulsed - field gel electrophoresis.

The molds therefore were made from agarose so that cells wouldn't stick to the sides or bottom.

To imitate the cactus root and its outer covering, they made a material composed of cellulose fibers, agarose cyrogel and microparticles.

First, they 3 - D - printed wormlike strands of a gel called agarose, each serving as a cast of a tiny blood vessel.

Cell extracts (1.5 mg) were incubated with 15 μL of anti-FLAG M2 - agarose affinity gel (Sigma - Aldrich, St. Louis, MO).

(This is an updated version of the current DNA Fingerprinting field trip, replacing agarose gels with the DNA chip.)

Using acrylamide instead of agarose gives much sharper bands.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).

Performed various tasks in the lab such as preparing agarose gels and buffer dilutions for electrophoresis.

State - of - the - art DNA chip analyzers can resolve DNA sizes far more accurately than possible with agarose gel electrophoresis.

He and his colleagues wanted to avoid the classic light - sheet arrangement in which the sample is embedded in a tube of agarose and surrounded by lasers and cameras.

The SLC6A4 promoter VNTR polymorphism was genotyped by agarose gel size fractionation as described in ref.

DNA integrity is assessed on agarose gel.

The transparent chamber contains agarose, a hydrogel with a texture that can be adjusted to various degrees of «hardness.»

However, this diving behavior is suppressed when the agarose contains food.

If yeast or sugar has been mixed with the agarose, they stay close to the surface of the hydrogel.

Then they carefully tugged or suctioned away the agarose casts, leaving channels that they lined with cells to create simple blood vessels.

After cells held in suspension in an agarose solution are grown around the vascular structure, a solvent can be used to wash the sugar away.

Amplified libraries were size - selected on a 2 % agarose gel at approximately 350 bp average length and then sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for deep sequencing on an Illumina Genome Analyzer IIx (Illumina, San Diego, CA).

After the RT - PCR process, samples are dyed and a few microliters from each tube are injected into different lanes of a gel made with agarose (a polymer derived from seaweed).

Following PCR amplification the PCR product was analyzed on a 2 % agarose gel and then extracted and gel purified using Wizard SV Gel and PCR Clean - Up System (Promega).

Conditions for the PCR reaction were 40 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s. Amplicons were purified on a 2 % agarose gel, cloned into plasmid vectors using TOPO TA (Invitrogen, Carlsbad, CA), and sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for Sanger sequencing in both directions using vector primers M13F and M13R.

Amplified sequences were visualized by gel electrophoresis in 2 % agarose gels stained with GelRed (Biotium).

When an electric current is applied along the length of the gel, the DNA starts to migrate through the agarose thicket.

For label - free CARS imaging the worms were anesthetized with levamisole and placed on a cover glass with a thin layer of 2 % agarose.

A 3 - ml overlay consisting of EMEM with 0.4 % agarose was added, and the cells were incubated at 37 °C for 72 hours.

If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands.