Sentences with word «confocal»
The word "confocal" means to have a common focus or point of focus. It often refers to a type of microscope or imaging device that uses a specific type of light to create high-resolution, 3D images of objects. It allows only a small focal plane of the specimen to be in focus at a time, improving clarity and reducing background noise. Full definition
The plankton molts were floating in seawater in a dish and imaged by laser scanning confocal microscopy.
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This image was created using a laser scanning confocal microscope by Igor Siwanowicz of Howard Hughes Medical Institute.
Using confocal laser microscopy and electron microscopy, coupled with the use of advanced synchrotron techniques at the Swiss Light Source, in Villigen (Switzerland), and at the Canadian Light Source in Saskatoon, Canada, the team was able to show that not only the morphology of microbial twisted structures is preserved after long incubations under diagenetic conditions, but also organic remnants can be detected in the mineralized twisted structures.
Druckenmiller houses a specialized laser scanning confocal microscope (the only one of its kind in the state of Maine), as well as a scanning electron microscope and a number of conventional fluorescent microscopes, all of research quality.
(Hayashi S. Resolution doubling using confocal microscopy via analogy with structured illumination microscopy.
ERG and CHD1 heterogeneity in prostate cancer: use of confocal microscopy in assessment of microscopic foci.
The unique location, large size, and specific arrangement of the Platynereis mesodermal midline cells allowed their unambiguous identification after whole - mount in situ hybridization (WMISH) and thus expression profiling by confocal imaging.
These models were tested with real measures of cell length in Arabidopsis roots, carried out with confocal microscopy in CRAG.
The chambers were fixed to the stage of an inverted spinning disk confocal microscope (Nikon, Andor), which was used to capture images of the cell populations residing on the coverslip glass.
Overcoming the limitations of confocal fluorescence microscopy, we invented and developed light sheet - based fluorescence microscopy (LSFM) establishing it as a major tool in the modern life sciences.
Kozlowski tackled that problem computationally; using the popular MATLAB programming environment, she and her colleagues developed an algorithm that classifies the shapes of microglia in confocal microscopy images with 90 % accuracy.
Secure RemoteCare service for Confocal Microscopy Systems.
They injected small polymer particles into each cell, then tracked their motions under a standard confocal fluorescent microscope.
Advanced Imaging Systems • Illumination Systems • Emission Systems • Spinning Disk Confocal Microscopy • Total Internal Reflection Microscopy (TIRF) • Light Sheet Imaging • Optogenetics
Current research efforts include investigation of the role of canine herpesvirus in spontaneous corneal disease in dogs and the effects of topical and systemic anti-inflammatory and chemotherapy drugs on its recrudescence; identification of novel infectious agents causing feline corneal disease; and the characterization of common and novel corneal disorders of dogs, cats, horses, and exotic species by in vivo confocal microscopic imaging.
A, Confocal Z - projection of 10 0.7 μm optical slices of geniculate ganglion from a 5 - HT3AGFP mouse showing expression of GFP (green) and P2X3 (magenta).
To learn how a clot contracts, the Penn team imaged clots (networks of fibrin fibers and blood platelets) using an imaging technique called confocal light microscopy.
Examples include imaging techniques ranging from confocal advances to cryotomography — which can resolve subcellular structure and reveal microbial cell function — and ways to monitor the production and exchange of microbial metabolites.
«Optical sections» or «Z Stacks» of samples can be obtained on confocal microscopes.
a-b) Representative high - magnification confocal micrographs depicting co-localization between pab27576 (red) and MOAB - 2 (green) in CA1 neurons of the hippocampus a) and neurons of lamina V of the parietal cortex b) at post-plaque stages (13 months).
Companies making high - content cell imaging equipment such as confocal microscopes and plate readers are now adding software tools to process images of 3D cell cultures.
Caroline joined the Biophotonics group as a post doc in 2013 to implement confocal light - sheet microscopy for structural whole mouse brain imaging and then later functional calcium imaging in Zebrafish.
«Furthermore, adapting in vivo corneal confocal microscopy for use in tracking HIV - induced PNS damage in patients may be of great value to identify early PNS damage independent of performing skin biopsies.»
As an appropriate ECM substrate for cancer cell migration, we use nonpepsinized rat tail collagen I polymerized at room - temperature and fluorescently labeled to facilitate visualization using standard confocal microscopes.
Additionally, high magnification confocal analysis revealed that the remaining hemocytes in these animals appeared morphologically identical to those overexpressing Bax (Figure 3F).
In this webinar, the principles of state - of - the - art confocal Raman imaging as a tool for the analysis of molecular characteristics of a sample will be presented, then the manner in which this information can be linked to structural information acquired with scanning electron microscopy will be demonstrated.
Inset shows higher resolution confocal images of photoreceptor cell nuclear layers (DAPI blue) and rhodopsin expression (red) in the dystrophic control (left inset) and dystrophic with iPS - RPE transplant (right inset) RCS rat.
The system's advanced confocal technology enables researchers to capture super resolution images with excellent clarity.
Multiple cell layers require a moderate axial resolution but still a high frame rate, which is provided by a spinning disc confocal fluorescence microscope.
Confocal analysis revealed that apoptotic hemocytes are very different in morphology to those exposed to Mcf1 appearing hugely swollen and containing fluorescent puncta having engulfed their dying neighbouring hemocytes (Figure 3E).
Our modern imaging center is equipped with state - of - the - art confocal laser scanning microscopes, fluorescence and stereo microscopes, two photon microscope, laser microdissection microscope and powerful image analysis computers with imaging software.
But manipulating large 3D confocal data sets requires special workstations, and even so, the rendering of a single tiled image is slow.
To address this need, here we use Drosophila embryos, specifically embryonic hemocytes, as models for studying the early stages of infection in real - time using time lapse confocal microscopy.
The ligand was pipetted directly into the bath in a volume of 100 μl to yield a final concentration of 10 — 9 M. Imaging was performed with a microscope (Axio Imager.A2, Carl Zeiss) equipped with a spinning disc confocal head (CSU - W1, Yokogawa).
The spinning disk confocal optical system acquires images up to 200 frames per second, capturing fast intracellular dynamics within live specimens.
It is also the place where confocal users will post reservation changes or cancellations.
All panels are z - projections of confocal stacks, ventral view, anterior up, white dashed line outlining foregut.
He studies DNA and RNA quadruplexes, nucleic acid structures that were first visualized via confocal microscopy in fixed, dead cells.
We then combined confocal microscopy and electrophysiology to explore the interactions between temperature, adrenergic stimulation and contraction frequency on the temporal and spatial properties of Δ [Ca2 +] i, and the excitation profiles of AP and ICa in isolated myocytes.
Additionally, our doctors are able to view tissue cells in the body at a microscopic level, using a technique called probe - based confocal laser endomicroscopy (pCLE).
See a snapshot of where confocal microscopy is today and a glimpse at where we might be in the future.
Embryonic hemocytes lend themselves beautifully to live imaging studies since fluorescent probes can be expressed specifically in these cells using hemocyte specific promoters and their movements subsequently imaged within living embryos using confocal timelapse microscopy.
Mapping the entire macaque limbic system using a 20 × confocal lens will require massive data sets — well over 600 terabytes per animal.
Korenberg's team is developing a 3D coordinate system to align various types of neuroimaging data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal data and, for some areas, subcellular resolution with electron microscopy.