Sentences with word «retroviral»
The word "retroviral" refers to a type of virus called a retrovirus. A retrovirus is a kind of virus that can change its genetic material by copying it backwards into the DNA of its host. This ability helps the virus to replicate and spread within the host's body. Full definition
This group is well known for its design of retroviral vectors for efficient gene transfer and it seemed like a good idea for me to go there for a year and exchange knowledge and expertise.
sciencemag.org
An experimental system for the evaluation of retroviral vector design to diminish the risk for proto - oncogene activation.
These cells were immortalized by retroviral transduction of the SV40 T antigen and Telomerase reverse transcriptase (TERT), the catalytic subunit of the telomerase complex [4], and then termed HMLE (Human Mammary with Large T and TERT).
«The integration of retroviral DNA is a relatively uncommon event compared with the number of viral DNA copies found in infected cells.
Molecular and cell biology of retrovirus - host cell interactions — Development and application of retroviral gene transfer and drug screening systems
The most important aspect of health and wellness for a three month old kitten is to have a physical examination to be sure he is healthy (including healthy for anesthesia), be sure he is free from internal and external parasites, test for retroviral diseases, complete his vaccination series and review information about training, socialization, husbandry and nutrition.
Our in - house diagnostic laboratory allows us to perform blood - chemistry panels, complete blood counts, Heartworm and tick - borne disease testing, feline retroviral testing, urinalysis, urine cultures, cytology and more.
The cDNA library was packaged into retroviral particles and introduced into the J - Lat 6.3 cell line via infection (Table 1).
The study, «VlincRNAs controlled by retroviral elements are a hallmark of pluripotency and cancer» found that novel non-coding parts of the human genome known as vlincRNAs (very long intergenic, non-coding RNAs) triggered by ancient viruses, participate in the biology of stem cells, and in the development of cancer.
The Jaskólski - Wlodawer team was recognized for structural studies on retroviral proteins, which have contributed to the development of new therapies for diseases such as AIDS and leukemia in children.
Elsässer SJ, Noh KM, Diaz N, Allis CD, Banaszynski LA, «Histone H3.3 is required for endogenous retroviral element silencing and genome stability», Nature (2015)
Dr. Jim Richards was leading the team of experts preparing this update on retroviral infections in cats when he suffered a fatal accident.
Only a few years ago, experts were increasingly optimistic about the fight against AIDS, as retroviral drugs were cheaper and awareness seemed to be rising.
Publication:» Histone H3.3 is required for endogenous retroviral element silencing in embryonic stem cells», Simon J. Elsässer, Kyung - Min Noh, Nichole Diaz, C. David Allis, Laura A. Banaszynski, Nature, online April 4, 2015, DOI: 10.1038 / nature14345.
My goal was to design retroviral vectors with which two transgenes would be expressed at the same time in equal amounts.
They assemble themselves like Lego blocks into bulbous retroviral particles, which ooze from the cells producing them.
His research focus is on the mechanisms by which endogenous cellular proteins impact retroviral replication, in particular the interaction of cellular TRIM5α with retroviruses such as HIV - 1.
In this study, we develop a reproducible protocol for efficient reprogramming mouse neural progenitor cells (NPCs) on human foreskin fibroblast (HFF) cells via retroviral transfer of human transcriptional factors OCT4 / SOX2 / KLF4 / C - MYC.
«The ethical issue of retroviral transfer from pig to man (which is in theory facilitated from organs of the double knockout pigs) remains a major one.
(A) Expression of pluripotency markers from iPSCs (HiPSC - 1 control) generated following retroviral transduction of unsorted HUF1 cells.
Feline retroviral testing is commonly performed via a variety of in - house ELISA tests, now offered by various manufactures.
Sequence comparisons placed the telomerase proteins in the reverse transcriptase family but revealed hallmarks that distinguish them from retroviral and retrotransposon relatives.
The day after retroviral transduction, cells were grown in NSC media for four days following a change to hESC media to support iPS cell growth (Figure 2A).
PreXMRV - 1 and PreXMRV - 2 share 99.92 % sequence identity with XMRV, and retroviral recombination between them can generate XMRV.
The researchers looked at how much the immune system was able to come back after starting retroviral therapy.
In late 2009 at CDC headquarters in Atlanta, a virologist in the agency's retroviral branch, William Switzer, was attempting to replicate the XMRV finding in CFS.
Twenty years earlier, another scientist, immunologist Elaine DeFreitas of the Wistar Institute in Philadelphia, had discovered retroviral gene sequences in CFS sufferers.
If we can understand why insertion doesn't occur more often, it might lead to new drugs that prevent retroviral infection.
He previously spent 20 years in the U.S. Army's Medical Corps, heading its department of retroviral research.
With the help of my colleagues I started growing retroviral vector - containing plasmids in bacterial cultures, isolating the plasmid DNA and transfecting special producer cell lines with these plasmids.
The successful demonstration of the efficacy of the synthetic DNA mimic might lead to a new approach to the treatment of AIDS and other retroviral diseases.
At the end of the project, the transduction efficiency of the newly made retroviral vectors was tested in hematopoietic cells, and here my «old» techniques came in handy.
According to retroviral immunologist Judy Mikovits of the Whittemore Peterson Institute (WPI) in Reno, Nevada, xenotropic murine leukemia virus — related virus, or XMRV, was present in 68 out of 101 people with chronic fatigue syndrome, compared with only 8 of 218 healthy controls.
Sure enough, Perron saw faint traces of retroviral activity.
Scan any animal's genome, and one will even find «fossil» viral genes, remnants of ancient retroviral infections.
No common retroviral integration sites are found among multiple clones.
Harold Varmus, M.D., shared a Nobel Prize in Physiology or Medicine in 1989 with J. Michael Bishop, M.D. for their discovery of the cellular origin of retroviral oncogenes.
The generation of induced pluripotent stem (iPS) cells from mouse embryonic and adult fibroblasts by retroviral introduction of transcription factors (Oct4, Sox2, Klf4 and c - Myc) was first reported by Yamanaka and his colleagues in 2006 [1].
Telomere replication is mediated by telomerase, an RNA dependent DNA polymerase structurally similar to retroviral reverse transcriptases and viral RNA polymerases.
Seventy - two hours after retroviral delivery of 4 factors into the NPCs, ES cell - like colonies could be detected.