Sentences with phrase «brain slices»

Then they prepared mouse brain slices from a group of neurons in the dopamine production center, at 24 hours and every day thereafter.

She suggests coloring different parts of brain slices (from dead people) using specific dyes and viewing them under a microscope.

To understand more, she conducted experiments in acute brain slices, small pieces of brain tissue where neurons stay alive and maintain their physiological properties.

Still, testing brain slices might not represent what happens in the brain of a living animal.

In general, it has been difficult to preserve electrophysiologically viable motoneurons in brain slices from adult mammals.

Experimental data supplied by Dr Colin Davidson (St Georges, University of London, 2011) demonstrated that desoxypipradrol potently stimulated dopamine release from rat brain slices in vitro.

Using electron microscopy to look at thousands of ultrathin brain slices taken from awake and sleeping mice, they found that after sleep, the size of most synapses — specifically, the surface area where two neurons touch each other — shrank by about 18 percent.

«We can take a 1 - mm whole brain slice and zoom in on it,» Ellisman said, «and keep zooming up to the diffraction limit, to the intranuclei, to the neurons in the cerebellum.»

In order to characterize this possible imbalance, we performed in vitro brain slice whole patch - clamp recordings from the Q175 heterozygous knock - in HD mouse model expressing GFP under the control of the D2 receptor promoter to identify dSPNs (GFP - negative) and iSPNs (GFP - positive).

Studies of individual neurons in dishes of cultured neurons and in brain slices by co-first author Robert E. Stanley, a graduate student in the lab, revealed that neurons in the mutant mice had decreased numbers of dendritic spines, an important part of the synapses that neurons use to communicate with one another.

Li Gan, PhD, hopes to use the microscope to image firing cells in live brain slices, while Leor Weinberger, PhD, is interested in studying the processes by which a virus enters a cell in real time.

As shown previously in VTA - containing brain slices exposed to cocaine (Argilli et al., 2008), DA neurons showed significantly elevated potentiation of mEPSC amplitudes in PLE − / GFP + DA neurons in the presence of CM - MK801, which further demonstrates the lack of activity for CM - MK801 in PLE − neurons.

Analyzing brain slices of the mice, the researchers saw increased phosphates on the GluR1 receptors and an increased ability of these receptors to be recruited to synapses.

In the Finkbeiner laboratory, Linsley uses time - lapse imaging of neurons in culture, within organotypic mouse brain slices, and within live zebrafish to uncover the mechanisms in neurodegeneration that lead to neuronal death.

To analyze the rats» brains, the team extracted brain slices from depressed and normal animals, soaked them in voltage - sensitive dye, and prodded them with electrodes next to a high - speed camera.

Brain slices reveal small (left) and large (right) lesions to the orbital prefrontal cortex.

When brain slices were bathed in a stress hormone, IP3 - induced calcium signaling also increased.

To see it, Nägerl and colleagues coupled a diffusible, fluorescent dye with super-resolution microscopy, in this case stimulated emission depletion (STED) microscopy, to generate high - quality, 3D reconstructions of the tissue and spaces within brain slices kept alive outside the body.

His dream is to create the world's most complete open - access neuroanatomy library, featuring high - resolution digital images of whole human brain slices.

Using brain slices from DA receptor transgenic mice, we show that this is not the case.

When the team compared brain slices from the two groups of rats, they discovered that in the intact animals, more CREB molecules carried a phosphate group — a crucial step for their activation.

To illustrate the discovery for me, Roy called up an image of a magnified brain slice in the lab.

To address these different topics we are developing relevant animal models that will be used in combination with primary neuronal cultures, rodent brain slices, and brain tissues.

To investigate neocortex development, we use in utero electroporation, coupled to live imaging of embryonic brain slices (Fig. 2).

Postmortem brain slices can be «read» to determine how a mouse was trained to behave in response to specific sounds...

(D) For brain slices treated with CM - MK801, AMPAR - mediated mEPSC amplitude was increased in PLE − VTA DA neurons following cocaine treatment.

Axial brain slices and corresponding scatter plots showing correlations between (A) plasma glucose levels and VMPFC / ACC response to high - calorie food images; and (B) plasma cortisol levels and left and right insula / striatal response to high - calorie food images.

Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices.

The Soak The first step in preparing for the whole - brain slicing takes months: The organ soaks in formaldehyde until it becomes rubbery.

These findings were confirmed by two - photon imaging of neurons in the brains of living mice by the lab of collaborator Yi Zuo, PhD, a neuroscientist at UC Santa Cruz, as well as electrophysiological recordings from neurons in brain slices by the lab of collaborator Vikaas Sohal, MD, PhD, an associate professor of psychiatry at UCSF.

STED nanoscopy of actin dynamics in synapses deep inside living brain slices.

One limitation of the approach presented here is that the aqueous solubility of CM - MK801 (5 — 10 μM) make it less suitable for direct injection into the brain because of the requirement for elevated levels of co-solvents, such as dimethyl sulfoxide (DMSO)(> 0.1 %), which resulted in VTA - containing brain slices that were unsuitable for recordings.

In - vitro brain slice recordings of both the R6 / 2 and Q175 mouse models of HD have revealed a paucity of glutamatergic innervation of the striatum as well as alterations in intrinsic membrane properties of striatal medium spiny neurons (MSNs, exemplified by a large increase in membrane resistance (Rm) and a reduced rheobasic current (Rh).

So Yang Shi and her PhD advisor, neurologist David Holtzman at Washington University were in for a surprise when they peeked at a set of brain slices from mice engineered to produce tau pathology.

Hu's team then took brain slices from the mice in order to measure the electrical currents produced from mPFC neurons.

In brain slices from normal rats, only about 7 % of cells were the bursting type, but in rats bred to display depressionlike behavior, the number was 23 %.

Studies of brain slices have shown, for example, that adenosine binds to and interferes with receptors on cholinergic neurons, the alertness - controlling cells found mainly in the basal forebrain.

«The angle and thickness of a brain slice are very important, and the direction of a column can change even within a single slice, which could be the reason why they weren't noticed before,» says Hosoya.

My project was in neuroscience, working on a model of epilepsy using electrophysiological recordings from brain slices.

Annese holds a whole - brain slice from another amnesic patient.

Via the pipette a red dye diffuses into the cell, rendering them visible in the brain slice.

Below: The whole - brain slices in the boxes were treated with a stain that colors cell bodies a deep purplish blue.

In a brain slice, Olivia Masseck measures the activity of nerve cells in which she switches on their receptors using light stimulation.

If it is shorting out like a bad lamp, Zhang could add a drug to the liquid in which the brain slice floats.

No other researcher had found evidence that neurons can breach the wall between these regions, but developmental neurobiologist Pasko Rakic thought he saw hints of such a migration in brain slices he examined in 1969.

This brain slice from a human autopsy has taken on vivid color in the hands of a neuroscientist: green from infection by a lentivirus, red for neurons, blue for the nuclei of brain cells.

Applying rolipram to brain slices from sleep - deprived mice restored LTP.

When the scientists tried to trigger LTP in these brain slices with electrical stimulation or chemicals, they found that methods that fired up cellular pathways involving the molecule cyclic adenosine monophosphate (cAMP) didn't work.