Sentences with phrase «embryonic fibroblasts»
"Embryonic fibroblasts" refers to cells that are found in the early stages of development of living organisms, specifically embryos. These cells have the ability to transform into different types of cells in the body and play an important role in the growth and development of tissues and organs. Full definition
Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3 / 4, Sox2, and Klf4 and the other containing the c - Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras.
science.sciencemag.org
Cyclooxygenase - 2 (COX - 2) was highly elevated by capsaicin treatment in tumors and murine embryonic fibroblasts from TRPV1 / KO mice.
Induced pluripotency in chicken embryonic fibroblast results in a germ cell fate.
Latex bead labelling and comparative immunofluorescence analysis of the p62 subunit of the TFIIH was performed as described previously [16,17] using primary mouse embryonic fibroblasts at passages 2 — 5.
Pluripotent cells can be created by introducing transcription factor genes into mouse embryonic fibroblasts on a plasmid that does not integrate into the genome.
Finally, by introducing both nanodiamonds and gold nanoparticles into a single human embryonic fibroblast, we demonstrate temperature - gradient control and mapping at the subcellular level, enabling unique potential applications in life sciences.
Rassf1A null embryonic fibroblasts displayed an increased sensitivity to microtubule depolymerizing agents.
Valdivia submitted this image of mouse embryonic fibroblasts forming focal adhesions, points of contact of the cell with the extracellular matrix.
Shi, Y.; Desponts, C.; Do, J. T.; Hahm, H. S.; Scholer, H. R.; Ding, S. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small - molecule compounds.
The production of virus - free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine.
For this study, Guttridge, first author David J. Wang, who developed many of the study's concepts, and their colleagues monitored NF - kB activity during tumor development using mouse embryonic fibroblasts and two mouse models.
In vitro experiments used human colon carcinoma cells with and without MMP9 and mouse embryonic fibroblasts, which are connective tissue cells that make the extracellular matrix and collagen and play an important role in tissue repair.
Vermulst's study looked only at tissues from the heart, brain, and embryonic fibroblasts.
I prefer to culture my hPSCs with mouse embryonic fibroblasts (MEFs) but there are occasions when feeder free growth is required (e.g., nucleofection, viral transduction or karyotyping to name a few).
FINDINGS The researchers identified 32 known imprinted genes, but no new ones, implying that the list of imprinted genes in the mouse — at least in embryonic fibroblasts — is nearly complete, says Morison, who was not involved in the study (Nucleic Acids Res, 42:1772 - 83, 2014).
TEST CASE RESEARCHER Piroska Szabó, Associate Professor, Center for Epigenetics, Van Andel Research Institute ORGANISM Mouse METHOD RNA - seq In 2014, Szabó and colleagues reported how they'd used mouse embryonic fibroblasts to test whether the relatively new method of RNA sequencing could reveal known and novel imprinted genes.
With current methodologies, mouse embryonic fibroblast (MEF) cells have been used as a feeder layer to derive both mouse and human iPS cells.
Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).
When transplanted into blastocysts, mouse iPS cells derived from mouse embryonic fibroblasts can give rise to adult chimeras, which are competent for germline transmission.
Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.
Briefly, ES colonies were maintained on mitotically inactivated mouse embryonic fibroblasts (density of 60,000 cells / cm2) in media consisting of DMEM (Invitrogen cat.
Cells were stained in solution using a mixture of GCTM - 2 (mouse IgM), TG30 (anti-CD9, mouse IgG2a) and Thy1.2 - PE (to gate out any mouse embryonic fibroblasts, BD Bioscience, cat.