Sentences with phrase «luciferase expression»
The phrase "luciferase expression" refers to the process of producing and releasing an enzyme called luciferase. This enzyme, when present, emits light, making it useful for studying the activity of certain genes or tracking the performance of various biological processes in cells or organisms. Full definition
Nonetheless, CDDO - Me and HPP - 1014 still were able to activate HMOX1 E2 - dependent luciferase expression in the presence of mutant Bach1 proteins, indicating that the CP motifs in Bach1 are not critical for efficient derepression of Bach1 by an electrophile (Figure 6D).
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In contrast, the ability of both CoPP and HPP - 4382 to induce HMOX1 E2 ARE - dependent luciferase expression in the presence of the mutant Bach1 protein was sharply inhibited.
Cells were transfected with 320 ng pGL3 luciferase expression construct containing the 3 ′ UTR of human APC, 40 ng pGL4.74 hRLuc / TK Renilla luciferase vector (Promega, Fitchburg, WI), and 25 nM hsa - miR - 142 precursor or negative control precursor (Ambion).
His lab crossed the K - ras model to a ubiquitous promoter driving conditional luciferase expression only in cells that Cre has entered.
All three compounds were able to induce luciferase expression, demonstrating their ability to overcome Bach1 repression of HMOX E2 - dependent transcription (Figure 6C).
In these assays, both wild - type Bach1 and FLAG - hBach1 - AP4 - 7 efficiently repressed basal levels of luciferase expression.
When compared to unfractionated hu - PBMCs, the CD8 + - depleted hu - PBMC recipients show similar reductions in luciferase expression following IL - 15 superagonist treatment.
Five days later, the live, treated mice showed a significant reduction in total luminescent flux, and spleen lysates from them showed a 99 % reduction in luciferase expression compared to HIV - LucR infected but untreated controls.
To co-transfect pCMV - Neo-APC plasmids, cells were transfected with 200 ng TOPFlash or FOPFlash luciferase expression construct, 20 ng pRL - TK Renilla luciferase vector (Promega), 300 ng pCMV - Neo-APC plasmids, and 25 nM hsa - miR - 142 precursor or negative control precursor (Ambion).
Expression of HMOX1 E2 - dependent luciferase expression was determined in HepG2 cells co-transfected with an HMOX1 E2 - dependent reporter plasmid and a plasmid expressing FLAG - tagged wildtype Bach1 (Figure 6A).
Luciferase expression was markedly lower in cells expressing Bach1, indicating effective repression of HMOX1 E2 - dependent transcription by Bach1 (Figure 6B).
When spleens from infected mice were examined for sustained control of infection at later time points, however, luciferase expression had rebounded.
Luciferase expression can be quantified both in cell lysates and in live mice using a luminometer.