Sentences with phrase «gene of interest»

Genetic modification is a valuable research tool that helps rice breeders understand gene function and identify genes of interest.

We aim to develop a system that links gene discovery to signaling pathways and eventually to set up animal models of disease by manipulating genes of interest through mouse genetics.

During my time, more tools became available for plant breeders to transfer genes of interest and improve selection efficiency.

«The challenge has been how to direct certain therapies designed to manipulate genes of interest in specific cells without developing a specific drug carrier for each specific cell type.

Maintained viable bacteria colonies and cultures for all necessary strains; conducted purification and extraction of genomic DNA; performed PCR for genes of interest; ran protein expression and activity assays.

Vaxwave ® is based on lymphocytic choriomeningitis virus (LCMV) and in this vector the gene encoding the LCMV envelope protein, normally responsible for virus entry into target cells, has been deleted and replaced with a target gene of interest.

In contrast to traditional approaches, RNAScope ISH is significantly faster and highly sensitive, permitting researchers to not only detect but also quantify, with confidence, the expression levels shown by genes of interest.

«What we decided to do was take a completely unbiased approach where instead of targeting individual genes of interest, we would express randomized guides inside of the cell,» Lu says.

The last gene of interest was TGM5, which encodes a protein that stabilizes protein groups.

Rapidly find and highlight genes of interest including virulence factors and antimicrobial resistance genes.

CRISPR has allowed scientists to tag and light up endogenous genes of interest to better understand normal protein function.

The Bioinformatics group uses computational methods to analyse genome sequences, amino acid sequences, and gene expression data, both to identify new genes of interest and to determine their structure, function and role in the cell.

One particular gene of interest that Dr. Chin presented was HOXA1, a gene involved in development.

The capacity to genetically engineer mouse models of cancer by either knocking - out or knocking - in selected genes of interest affords the opportunity to follow in - vivo tumor progression (and regression) and metastases.

ΔCT was defined as Ct gene of interest − Ctβ - actin.

However, if the purpose of such a scan is to identify candidate genes, then — as with single - gene studies — more detailed follow - up and functional validation of at least some of the key genes of interest are necessary to shore up the evidence.

«We thought that if we could link signals from water molecules to the expression of genes of interest, we could change the way the cell looks under MRI,» says Arnab Mukherjee, a postdoctoral scholar in chemical engineering at Caltech and co-lead author on the paper.

Using genetic modification in this way can improve the accuracy of identifying genes of interest and speed up the breeding process, even though the end - product is not GM rice.

As a rule of thumb, plant breeders in both public research institutes and private companies use genetic engineering only in the following situations: 1) the gene of interest is not found within the crop gene pool, 2) if found in the gene pool, transfer will be difficult due to incompatibility and other breeding problems or 3) the transfer of the gene will take a long time as in the case of perennial crops like coconut.

It greatly increases the accuracy of incorporating only the gene of interest, and its associated trait, into a new rice variety.

Viral - mediated gene transfer now enables investigators to overexpress or knock out a gene of interest within highly localized regions of brain.

The gene of interest from L - asparaginase was cloned, and the researchers used genetic engineering to make E. coli express large amounts of the enzyme originally found in yeast.

There were about 40 genomes of bacteria sequenced at that time, and what you could do was compare your gene of interest to other genomes to see if they contained something similar.

To gain more insights as to why zebrafish are more successful at regenerating spinal tissue, the researchers analyzed the gene expression of fish spinal tissue following injury, identifying seven genes of interest.

«We therefore looked for patient characteristics that would account for this result; next generation sequencing (NGS) of the genes of interest was performed on the archival tissue samples from the patients» primary tumours,» explained Ciardiello.

The complex sits in the region of DNA of interest and promotes expression of a gene of interest.

In this case the gene of interest is one that makes Channelrhodopsin - 2 (ChR2), a photosensitive protein used by unicellular algae to help them move towards light.

Most tests require three groups: controls, genetically altered mice with one copy of the gene of interest, and genetically altered mice with two copies of that gene.

To visualize different cell types in the brain, researchers often need genetic access to that cell — the ability to express a gene of interest only in, say, chandelier cells, but not neighboring neurons.

Mutalik led the effort to design a set of promoters and RBSs that could be attached to any «gene of interest» such that a bioengineer could be near certain (well, 93 percent sure, but that's near certain in biology) that the protein could be made within an organism to a desired target range.

The big thing then (as now), Ruvkun says, was for researchers to demonstrate that a gene of interest exists in a spectrum of different species — from roundworms and fruit flies to humans.

ACE is the most studied and is still a gene of interest, but we're trying to figure out if it's important and how — and the same question is reflected in ACTN3, but not reflected in ads for the test.

When researchers want to study a specific gene, they often make slight modifications so a special protein tag that produces fluorescent light can attach to the proteins produced by their gene of interest.

The Finnish researchers had used a set of «gene wrenches» called RNAi molecules, to turn off genes of interest, and with these additional experiments the researchers identified tracks leading to a nuclear protein and gene controller protein called SRF.

«We have deposited all the data and the toolkit in a public platform, which will help researchers identify and validate the functions of their genes of interest,» says Auwerx, placing the project within the context of EPFL's ongoing efforts to advance open science.

A well - defined cell type that has been widely used to identify the consequences of ablation or ectopic expression of a gene of interest.

To address the editing challenges, we have developed a system that is based on the delivery of recombinant Cas9 protein complexed with an sgRNA targeting the gene of interest (Cas9 / sgRNA ribonucleoproteins [RNPs]-RRB- via cell - derived nanovesicles, called gesicles.

Gesicles contain active Cas9 protein complexed with an sgRNA specific to a gene of interest.

The Guide - it CRISPR / Cas9 Gesicle Production System contains everything you need to easily produce gesicles that efficiently target your gene of interest.

Your sgRNA for a gene of interest is cloned into the provided linearized expression plasmid, pGuide - it - sgRNA1.

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest.

The genes of interest are described in the manuscript linked below, many of which are involved in what would be considered «normal» angiogenesis.

The Guide - it CRISPR / Cas9 Gesicle Production System contains everything you need to easily produce gesicles that will efficiently target your gene of interest.

A single mouse click on a gene of interest will direct the investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the screenshot below).