Sentences with word «oligo»

(FODMAP is an acronym for fermentable oligo - di - monosaccharides and polyols, which is the name of the carbohydrate in question.)
Importantly, this requires a separate assay: usually, a custom array with oligos designed for each exon in every panel gene.
After DNase treatment, total RNA concentration was quantified and 1 μg total RNA was converted to cDNA using oligo dT and reverse transcriptase.
The most common source for oligos in the diet is wheat products.
A pair of oligos for each targeting site (Table S1) were annealed and ligated into linearized pX330 vector for generating gRNA - expressing plasmid.
RNA was DNase treated (Roche) and cDNA was reverse transcribed using Transcriptor (Roche) with oligo dT as the primer.
We provide tools for oligo order generation, sanger sequencing submission and review, CRISPR damage analysis and integration of standard tickecting systems for production narrative.
The data collected by microarray are based on the fluorescence signal intensities (analogue data) from known sequenced oligo probes.
The hope was that antisense oligos for everything from cancer to HIV would roll off the pharmaceutical assembly line — but despite a generation of valiant effort, the successes on this front have been meager.
About Sisterna... Sisterna B.V. is the market leader in high - grade sucrose esters and sucrose oligo esters.
About Sisterna ® sucrose esters... Sucrose esters of fatty acids, commonly known as sucrose esters and sucrose oligo esters, are a relatively new extension to the line of emulsifiers available in the US.
The primers in the KAPA kit target Illumina ® P5 and P7 flow cell adapter oligo sequences.
For droplet based data one simple reason for the heterogeneity could be due to variation in coverage for DNA oligos on barcoded beads.
Here we utilized BD ™ AbSeq, a novel protein sequencing approach enabled by oligo - conjugated antibodies, for simultaneous detection of mRNA and protein in single cells.
We put our starting molecule» — a structure called oligo - enediyne, composed of three benzene rings linked by carbon atoms — «on a silver surface, and then induced reactions by heating it.»
And we can only sell twelve of them a year because there's not enough oligos in the world.
A new Oligo - Miocene marsupial lion from Australia and revision of the family Thylacoleonidae.
Levels of APOE protein dropped by about half in mice given the APOE compound as compared with those that received the placebo oligo or saltwater.
Glioblastomas make up 55 - 60 percent of gliomas, with lower - grade astrocytomas comprising 15 - 20 percent of cases, oligodendrocytes 12 - 20 percent and combination oligo - astrocytomas at 5 - 10 percent.
Kole says antisense technology should also be better at restoring genetic function than knocking out oncogenes, which cause cancer, because oligo - nucleotides are often not 100 per cent effective.
After four rounds of selection, the enriched oligo pool was sequenced, and a position weighted matrix was generated for the 12 bp target site and one flanking residue per side (faded).
For validation, we might be looking at custom arrays or oligo sets in addition to 3730 and 454 sequencing.
The antibody - specific oligos were captured, amplified, and sequenced alongside mRNAs in a single workflow on the BD RhapsodyTM single - cell analysis system.
Here, we describe two recently developed applications that utilize antibody conjugated oligos to enhance existing scRNA - seq platforms.
Wender and Waymouth's system, called charge - altering releasable transporters, are made of initially positively charged oligo (α - amino ester) polymers that bind the negatively charged mRNA.
gRNAs targeting every gene in the human genome for example, can be made easily and cheaply using oligo library synthesis.
In addition, we build in - house oligo microarrays for ncRNA expression profiling, such as microRNA and tsRNA, ultraconserved and Pyknon ncRNA expression profiling.
Templates may be supplied as annealed oligos, uncut plasmid, linearized plasmid, or ds DNA fragments.
I live in Taiwan, and agave syrup is not available, but Oligo syrup is sold everywhere.
Reddy BS, Hamid R, Rao CV Effect of dietary oligo fructose and inulin on colonic pre-neoplastic aberrant crypt foci inhibition.
La Sal restaurant offers avante garde international cuisine, outdoor dining, Oligo restaurant offers classic Mediterranean breakfast recipes, Air Ibiza rooftop terrace with pool and panoramic views, Alabastro Lounge Bar with tapas / snack menu, and Wellness bar.
FODMAP = Fermentable Oligo - Di - Monosaccharides and Polyols.
cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA).
RNA was isolated from cancer cells using the RNeasy RNA isolation kit (Qiagen), and complementary DNA was synthesized using oligo (dT) primers and Moloney murine leukemia virus reverse transcriptase (Invitrogen).
The mice that received the APOE antisense oligos had about half as many amyloid plaques as mice given saltwater.
An «oligodynamic effect», from the Greek oligos (few) and dynamis (power), refers to potent and meaningful biological actions that can be exerted by extremely low concentrations of metal ions.
Original was Oligo dT (24).
I've noticed that you include almonds and almond butter with no limit in your chart of foods that are okay, however Sue Shepherd (the pioneer of FODMAPs) says no more than 10 or it will contain high amounts of Oligos.
He brought it into Illumina, got it working, licensed it out to the oligo manufacturers, brought the price of oligos down by like 90 % and dramatically increased supply» — which enabled the now — $ 24 billion - in - market cap Illumina to succeed.
FODMAP is an acronym for: Fermentable Oligo - saccharides Di - saccharides Mono - saccharides And Polyols FODMAP's are sugars that are poorly absorbed by the body which can lead to IBS symptoms.
SEPPIC offers a range of innovative ingredients composed of nutritive elements (minerals & oligo - elements), natural extracts (polyphenols, antioxidants, polyunsaturated fatty acids) and coating agents.
Sometimes lactobacilli need a bit of help getting hold in the intestines, and some practitioners recommend FOS (fructo - oligo - saccharides) to enhance colonization.
In a new study, patients who ate rye bread that was low in so - called «FODMAPs» (fermentable oligo - di - and mono - saccharides and polyols) experienced milder IBS symptoms than patients who ate normal rye bread.
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