Sentences with phrase «target sequence»

Essentially, the process uses genetic techniques to target sequences of DNA inside the mutant gene.

Yet I can also tell you that they're inherently noisy — particularly for targeted sequencing data — and I would not be confident in using them to guide clinical care.

(A) Gene mutations identified by targeted sequencing in 125 patients with peripheral T - cell lymphomas.

Identification of Functional Variants for Cleft Lip with or without Cleft Palate in or near PAX7, FGFR2, and NOG by Targeted Sequencing of GWAS Loci The American Journal of Human Genetics DOI: 10.1016 / j.ajhg.2015.01.004

We used the donation to design a huge set of primers that we can use to make dozens more mitochondrial targeting sequences (MTSes) to test for their ability to target proteins to the mitochondria.

Each of Glo's RNA - binding domains therefore contains two distinct binding surfaces that interact with different types of RNA target sequence.

«We've constructed variants of the TAL proteins which simultaneously recognize two custom target sequences at different sites in the DNA and then basically staple them together,» says Dietz.

Sarah Weckhuysen and colleagues report a study based on targeted sequencing of the genes encoding the components of the GATOR1 (DEPDC5, NPRL2, and NPRL3) and GATOR2 (MIOS, SEC13, SEH1L, WDR24, and WDR59) complex in European probands with focal epilepsy with or without focal cortical dysplasia.

On this front, Yildiz, working with CRISPR - Cas9 co-discoverer Jennifer Doudna, also at Berkeley, on August 4 described in Science Advances why CRISPR - Cas9 cuts at specific target sequences in the genome, and where the tendency for off - target binding comes from.

There, some of the added DNA was swapped with the matching target sequences in the cells» genomes.

Yet, despite the system's effectiveness in finding and cutting target sequences in the genome of many organisms, unspecific activity that lets Cas9 randomly cut at secondary, not completely identical sites still poses a problem to genome engineers.

Jennifer Doudna and Samuel Sternberg used a combination of single - molecule imaging and bulk biochemical experiments to show how the RNA - guided Cas9 enzyme is able to locate specific 20 - base - pair target sequences within genomes that are millions to billions of base pairs long.

After earlier working with high - resolution CGH arrays, Levy's group now uses targeted sequence data.

Previously, we have described a number of practical considerations that should be taken into account when deciding where to place an FP within a fusion construct [1,2], i.e. positioning of the FP sequence relative to cell compartment targeting sequences, which often have absolute position requirements.

The spacer regions, on the other hand, are the unique, foreign DNA target sequences specific to each individual crRNA.

The Cas9 enzyme is guided to its genomic target sequence by a small guide RNA with a complementary sequence.

More targeted sequencing of additional tumors was conducted to track how instructions encoded in DNA were translated into the proteins that do the work of cells.

The PCR reactions occur inside the individual droplets, which the machine then sorts with fluorescent markers to detect target sequences.

The first step involves tagging target sequences with fluorescent markers.

Fully implemented targeted sequencing - based assays in routine diagnostic pathology laboratories are currently lacking in lymphoid cancer care,» explained Christian Steidl, MD, Senior Scientist at the BC Cancer and Associate Professor in the Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.

Targeted sequencing improves Neandertal mitochondrial DNA retrieval and reveals low diversity among individuals.

To determine how tightly the probe binds, researchers heat it up to shake the probe loose; however, often there's only a few degrees difference between the «melting» temperature of a probe bound to the exact target sequence and one where there is a minor discrepancy.

The first involves the primer - mediated enzymatic amplification of specific beta - globin target sequences in genomic DNA, resulting in the exponential increase (220,000 times) of target DNA copies.

Precision drives could exclusively affect particular species or subpopulations by targeting sequences unique to those groups.

One ZFN pair was used to target both the human and rhesus macaque CXCR4 genes since the 24 bp target sequences are identical.

Immunizing drives could block the spread of unwanted gene drives by preemptively altering target sequences.

The projects were still ambitious: large - scale targeted sequencing of deadly tumors, like lung cancer and glioblastoma.

Targeted sequencing experiments yielded a large number of high - quality genetic variants in both humans and dogs.

Targeted sequence alteration in the genomes of mammalian cells mediated by oligonucleotides

Whole Exome Sequencing (WES) on all samples (as compared to targeted sequencing panel or other more limited testing)

Chi's presentation focused on first - line treatment outcomes and correlations with deep targeted sequencing of circulating tumor (ctDNA) that occurred at baseline.

We tested if our bacterial derived components could be assembled for plant function by adapting each component with eukaryotic targeting sequences.

Our discovery that this conservation also extends to the level of HK transmembrane signaling allowed us to design a test system, whereby transmembrane fusions can be rapidly developed and tested in bacteria and then, with the addition of proper targeting sequences, function in plants.

They next undertook targeted sequencing of the lysine - 27 residue in all 16 genes that code for H3 isoforms in a validation cohort comprising 43 DIPGs and 36 non-brainstem glioblastomas.

With SapTrap, the user first designs either oligos or synthetic DNA for the desired gRNA target sequence, as well as the 5» and 3» homology arm repair template (Fig. 1, Step 1).

There is no need for PCR or cloning, as digestion of the destination vector with SapI yields 2 sites - the first site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology arm repair template.

First observed in B - cell lymphoma, recurrent mutations of epigenetic modifier genes have recently been identified in PTCL - NOS. 9 In the present study, we performed targeted sequencing of the main epigenetic modifier genes in a large cohort of Chinese PTCL - NOS patients.

The NTRK fusion genes were identified in part through targeted sequencing of RNA.

«Simultaneous mutation detection, copy number measurement, and digital gene expression profiling of high - grade serous ovarian cancer FFPE samples using Hyb & SeqTM targeted sequencing technology»

Too often, I see a paper with «Whole - genome sequencing» in the title in which a handful of samples actually obtained WGS, and the rest got targeted sequencing.

«Hyb & SeqTM Technology: A no - amp, no - library, single - molecule targeted sequencing approach developed for clinical applications»

Researchers from 2 published journals found that the 7 Day Ab Targeted sequence, performed just 3 times a week, produced the same results as 3 long duration (the slow boring stuff) cycling sessions, each of which lasted 90 minutes to 2 hours!

Therefore, Teitell's group opted to further modify their constructs with a 3 ′ - UTR mitochondrial targeting sequence (MTS) from human mitochondrial ribosomal protein S12 — the same essential approach used to optimize the import of allotopically - expressed proteins by Dr. Marisol Corral - Debrinski, (3,4) and subsequently advanced for multiple additional ETS subunit proteins by Dr. Matthew O'Connor's group at the SENS Foundation Research Center (RC).

In my talk I will highlight recent advances and applications of long - read PacBio sequencing in this field, both for targeted sequencing as well as shotgun metagenomics, metatranscriptomics and metaepigenomics.