Sentences with phrase «blot analysis»

Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho - PDGF Receptor (Tyr754)(23B2) Rabbit.

To further increase the sensitivity and specificity of the PCR assays, amplified gag and pol sequences were confirmed by Southern blot analysis using the biotinylated oligoprobes XGAGP2 (5» ACC TTG CAG CAC TGG GGA GAT GTC 3»), and XPOLP (5» TTG ATG AGG CAC TGC ACA GAG ACC 3») and chemiluminescence detection.

Western blot analysis showed high expression levels of all mTOR signaling proteins in both untreated ALCL cell lines.

BIOMED - 2 multiplex immunoglobulin / T - cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics.

Additionally, Western blot analysis indicated that the expression level of p21waf1 / cip1 gene was also enhanced by the ectopic expression of DDX3 in HuH - 7, HeLa, and 293T cells (Fig. 2B).

Southern blot analysis confirmed target gene deletion.

RNA blot analysis revealed a messenger RNA of four kilobases with highest amounts in brain and spleen.

Genomic DNA blot analysis also suggests that beta - ARK may be the first sequenced member of a multigene family of receptor kinases.

Western blot analysis revealed high levels of p - mTOR associated with high levels of p - p70S6K, p - rpS6, p - 4E - BP1, and total eIF4E in both cell lines (Fig. 1).

Western blot analyses with phospho - specific antibodies suggested that stretching induces phosphorylation of ERK of the MAP kinase pathway, but did not induce phosphorylation of phosphatidylinositol 3 - kinase.

Western blot analysis following treatment with rapamycin (B) or transient transfection with mTOR siRNA (C) showed increased levels of the CDK inhibitors p21waf1 and p27kip1, underphosphorylated retinoblastoma protein, and decreased cyclin A in a dose - dependent manner.

A, Western blot analysis after subcellular fractionation showed that Karpas 299 and SU - DHL1 cells express phosphorylated mTOR, 4E - BP1, and rpS6 as well as total eIF4E predominantly in the cytoplasm.

For tau deposition, Braak stage was IV / VI, and the Western blot analysis score was 9c / 10.

Western Blot analysis of LGALS3 expression in human stomach.

Western blot analysis showed almost complete silencing of AKT1 gene product that was associated with decreased levels of p - mTOR (C).

Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies.

(C) A shorter centromere is observed in progeny strain MN89 based on Southern blot analysis using restriction enzymes SwaI and HpaI.

Moreover, in situ hybridization and RNA blot analysis indicate that the 5HTlc receptor is expressed in neurons in many regions of the central nervous system and suggest that this subclass of receptor may mediate many of the central actions of serotonin.

Western blot analysis confirmed adequate inhibition of protein expression in transiently transfected cells.

The majority of t (1; 19)(q23; p13)- carrying leukemias and cell lines studied contained rearrangements of E2A as determined by DNA blot analyses.

(A) Western blot analysis for ApoB and ApoC - III in pooled TRLs (5 μg) isolated from fasted Ldlr — / — Ndst1fl / fl Alb - Cre + mice treated for 4 weeks with control or ApoC - III ASO (n = 3 — 4 / pool).

EdiGene Knockout Cell Lysates can be used to validate antibodies for specificity or used as high - quality negative controls for Western blot analysis.

However, all these studies involved only western blotting analyses of crude not cell - fractionated muscle homogenates.

These results were also confirmed by Western blot analysis (Fig. 2C).

C, Western blotting analysis of KLF4 in the same set of CSCs as described in (B).

Northern blotting analyses were performed on control and 3 h hypoxic samples to confirm that mRNA levels for P0 remained unchanged during hypoxia.

Methods: Rat neonates and AEC II from premature rats were exposed to hyperoxia in the absence or presence of CGRP, Wnt7b and β - catenin protein levels were measured by Western blot analysis, while T cell factor (TCF) and c - myc mRNA levels were determined by reverse transcription - polymerase chain reaction (RT - PCR).

Western blot analysis of Beta - actin in various tissues and cell lines using Proteintech antibody 60008 -1-Ig at a dilution of 1:5000.

Phosphorylated Akt and total Akt in insulin - treated cells (+) and untreated controls -LRB----RRB- were quantified by Western blot analysis.

Data was kindly provided by Ann Hammarstedt (Department of Molecular and Clinical Medicine, Gothenburg University, Sweden), who performed the Western blot analysis of Akt proteins, incubation of cells in MCD - media, and subsequent qRT - PCR analysis.

Western blot analysis of TUBB3 in various cell lines with 66375 -1-Ig (TUBB3 - specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band.

Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies.

Western blot analysis of TUBB3 in various tissues and cell lines with 66375 -1-Ig (TUBB3 - specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours

Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004 -1-Ig at a dilution of 1:10000.

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using the anti-HA antibody.

SN designed and performed the Western blot analysis and helped to design and perform tumor - sphere formation assays.

Western blot analysis confirmed that this was indeed the case.

Western blot analysis was done using standard methods as described previously (21).

Expression of adeno - myrAkt in infected cells was confirmed by Western blot analysis using a monoclonal antibody specific for the HA tag.