Sentences with word «efflux»

The findings, published November 28 in Scientific Reports, offer a better understanding of the motions and functions of efflux pumps.

We thought, if the latter scenario is operative, then a molecule comprising the minimal portion of the ADEP that is recognized by the pump could competitively interfere with efflux of the ADEP.»

This hypothesis was supported by our observation of increased levels of expression of several influx transporters (Oat2, Oct3, Octn1, and Oatp3) after 4 weeks and again at 16 weeks, positioning their changes before and during the increases in efflux transporters (Abcb1, Abcb8, Abcb9, Abcc5, and Abcc10) that occurred mainly at 8 and 16 weeks.

In this study, «we argue that when the acidified lysosome is depolarized by a sodium channel, it results in calcium efflux from lysosomes via the calcium channel encoded by the cacophony gene,» they wrote.

Heterogeneity in a bacterial population that arises through a mechanism of biased partitioning of drug efflux pumps, as we identified in our study, could be a stepping - stone on the path of bacterial populations towards antibiotic resistance.»

This picture was suddenly complicated in 1999, when Gerd Jürgens and his colleagues at the University of Tübingen in Germany discovered a mutant of the model plant Arabidopsis with efflux pumps everywhere in the cell.

For those that are not having significant changes by 3 months then I am adding Biocidin to remove biofilms and to decrease antibiotic resistance by blocking efflux pumps that remove antibiotics from germs.

New supercomputer simulations have revealed the role of transport proteins called efflux pumps in creating drug - resistance in bacteria, research that could lead to improving the drugs» effectiveness against life - threatening diseases and restoring the efficacy of defunct antibiotics.

«By understanding how the pump moves and dynamically behaves, we can potentially find a way to deactivate the pump — and antibiotics that haven't worked in a long time may be useful again,» said Los Alamos biophysicist Gnana Gnanakaran, who collaborated with colleagues at the Laboratory and with bacterial efflux pump experts Helen Zgurskaya at the University of Oklahoma and Klaas Pos at Goethe University in Frankfurt, Germany.

This results in the accumulation of the cytotoxic drug in cells with disabled efflux mechanisms and reduces resistance.

The TB bacteria use efflux pumps as a strategy to remove drugs and other threats from the cell before they can work against the bacteria.

Second, our current study suggests that after the long - term drug administration in mice, the more rapid recovery may also involve the interplay of ABC efflux transporters and SLC influx transporters in the testes.

This intrinsic penetration barrier, combined with potential for rapid efflux often leads to where a compound with good intrinsic activity at an intracellular or periplasmic target displays poor whole cell antibacterial activity.

In - vitro cell culture assays were used to analyze the effect of MIA - 602 on efflux pump function.

Kim Y, Park S - J, Lee B - Y, Risk D (2016) Continuous measurement of soil carbon efflux with Forced Diffusion (FD) chambers in a tundra ecosystem of Alaska.

One of the primary reasons cancer cells develop resistance is the overexpression of what are known as efflux pumps — proteins that protect a cell by pumping out unwanted toxic substances before they can reach their intended target.

The AcrA - PPMO also was effective against the human pathogens Klebsiella pneumoniae and Salmonella enterica, since those bacteria contain the same efflux pump with a matching gene sequence, Dr. Greenberg said.

This is the first time researchers have tailored one of these synthetic compounds, part of a class called PPMOs or peptide - conjugated phosphorodiamidate morpholino oligomers, to target a specific efflux pump found in bacterial cell walls, said Dr. David Greenberg, Associate Professor of Internal Medicine and Microbiology at UT Southwestern and a senior author of the study.

In fact, the team showed that the fragment approach worked better than combining ADEPs with reserpine, a well - known efflux inhibitor.

Concomitantly, the up - regulated efflux transporters situated at the basolateral membrane of Sertoli cells could then enhance transport of the drug out of Sertoli cells against a concentration gradient and, perhaps resulting in enhanced efficiency of recovery of spermatogenesis upon cessation of drug treatment.

Specifically, these investigators showed that the levels of several efflux (P - glycoprotein, MRP1, ABCG1) and influx (SLC15A1) pumps were found to increase by several fold within hours of adjudin treatment (27).

«In the fasted state there is a net amino acid efflux from muscle, i.e., a catabolic state.

To check for the presence of this subpopulation of SP cells, we exposed HCT116 to increasing concentrations of Bleomycin and performed Hoechst efflux assay.

Ceruloplasmin has been proposed as an important factor in cellular iron efflux and has been suggested to be downregulated in atherosclerotic plaques.3 Ceruloplasmin functions as an antioxidant.

MIA - 602 also suppressed efflux pump function in both cell lines.

The innate resistance of Gram - negative bacteria to antibiotics and other antimicrobials is a consequence of combinatorial effects of their ability to efflux antibiotics out of the cell and their capacity to form antibiotic tolerant biofilms.

It had been reported that the ADEP resistance of M. tuberculosis was due to the presence of one or perhaps more efflux pumps.

He also mentions that statins do not increase efflux capacity.1

The UTSW experiment focused on Escherichia coli (E. coli) and bacterial pathogens with genetically similar efflux pump complexes.

A promising approach for treating cystic fibrosis is the activation of the calcium - activated chloride channel TMEM16A as an alternative route for chloride efflux.

«There are two scenarios for how an ADEP efflux pump could operate,» Sello said.

Some life - threatening infections do not respond to antibiotics because efflux pumps inside a particular type of infectious microbe called Gram - negative bacteria flush out antibiotics before the drugs can work.

Some coded for multi-drug efflux pumps, which are multi-drug resistance mechanisms embedded in the bacterial membrane that pump specific antibiotics out of the bacteria.

One cell, the mother cell, keeps an old cell pole that originated in a past division, where efflux pump proteins have formed stable clusters.

Saint Louis University researchers then synthesized structural analogs of the discovered efflux pump inhibitors and identified properties essential for their activities.

Although some antibiotics can permeate the protective barriers surrounding bacterial cells, many bacteria have evolved efflux pumps that expel antibiotics back out of the cell and render the medications ineffective.

Our observations and models demonstrate an unexpected influence of surface - generated mesoscale eddies in the transport of hydrothermal vent efflux and of vent larvae away from the northern East Pacific Rise.

There is an urgent need to quantitatively measure and image the localisation of antibacterial agents in bacteria and biofilms and to understand penetration and efflux processes.

Laminin cultured cells arrange themselves in lobule like structures, express MRP1 and MRP2 and are capable of biliary efflux and show significant effect on hepatocyte P450 enzyme metabolic activity.