Sentences with phrase «gene knockout»
"Gene knockout" refers to a scientific technique where a specific gene is intentionally deactivated or removed from an organism's DNA. This allows researchers to study the effects of that gene's absence and understand its role in the organism's traits or functions. Full definition
These studies from the Capecchi and Smithies laboratories provided one of the essential ingredients for constructing gene knockouts in mice, the ability to target genes in cultured animal cells.
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In this study, by using a systemic mtEF4 gene knockout mouse model, researchers found that mtEF4 knockout damages the oxidative phosphorylation function in germ cells of male mice, thus causing male sterility.
Meehan et al., (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium.
Kohli, Manu, et al. «Facile methods for generating human somatic cell gene knockouts using recombinant adeno ‐ associated viruses.»
Yeast, bacteria, worms, and flies had long been used for gene knockout studies, and mice with knockout genes had just been developed before Waldman began his experiments.
Although protein is highly conserved in evolution, previous mtEF4 gene knockouts in some model organisms did not show significant phenotypic change.
«Under this grant, we will utilize Lexicon's proprietary gene knockout technology to study genes that may impact the function of motor neurons to advance treatments for spinal muscular atrophy,» said Brian P. Zambrowicz, Ph.D., executive vice president of research at Lexicon.
Citation: Ni W, Qiao J, Hu S, Zhao X, Regouski M, Yang M, et al. (2014) Efficient Gene Knockout in Goats Using CRISPR / Cas9 System.
Aside from single gene knockouts to allow for metabolic selection systems, the CHO host itself remains largely unchanged from that which was used thirty years ago.
Here, we report that CRISPR / Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts.
Secondly, they enable the use of animals with physiology more similar to humans rather than mice, which are the animal of choice for most mammalian gene knockout models.
Though the technique won't replace gene knockout strategies, in which an animal is engineered to lack a certain gene entirely, RNAi is faster and easier.
Finally, they allow the in vivo investigation of proteins where gene knockout leads to embryonic / neonatal fatality.
The pharmacological creation of such models («chemical genetics») has a number of advantages over classical gene knockouts.
Bouché N and Bouchez D (2001) Arabidopsis gene knockout: phenotypes wanted.
According to Dr. Burgess, the team is now working to extend the period that sperm cells can be cultured, so there is more time to select cells that have the foreign gene inserted in specific areas of the genome in what is commonly called a «targeted gene knockout.»
Researchers are using CRISPR - Cas technology to generate more accurate and efficient models of disease in cell lines, primary cells, and animal models through gene knockout, DNA sequence insertion, or gene up - or down - regulation.
Our CRISPR / Cas9 system is a simple, RNA - programmable method to mediate genome editing in mammalian cells; it can be used to generate gene knockouts (via insertion / deletion) or knockins (via HDR) in hiPS cells.
Continue to develop clinically relevant preclinical chemoprevention models through the prevention lab (e.g., tissue / organ gene knockout models)
Programmable nucleases, ZFN, TALEN and RGENs enable gene knockout in cultured cells and organisms by producing site - specific DNA double - strand breaks, whose repair via error - prone non-homologous end joining (NHEJ) or microhomology - mediated end joining (MMEJ) gives rise to frameshift mutations.
For example, Kuroiwa et al. reported that it took upwards of 21.5 months or longer for producing double - gene knockout cattle [34].
In this study, we demonstrate for the first time that Cas9 / gRNA - mediated gene knockout approach is highly efficient in goats resulting in successful generation of cloned goats with biallelic mutations.
Recently, Hai et al. reported generation of single - gene knockout pigs by zygote injection of CRISPR / Cas system [13].
The single - gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in live - born goats harboring biallelic mutations.
Other groups in England and Japan were also working on creating gene knockouts in human cells, targeting other genes.
Indeed, several lines of evidence suggest that cytokines are associated with animal and human aggression [102]--[104] and IL - 6 was causally linked to aggression in mice by gene knockout evidence [105].
Dopamine supersensitivity occurs in schizophrenia and other psychoses, and after hippocampal lesions, antipsychotics, ethanol, amphetamine, phencyclidine, gene knockouts of Dbh (dopamine
Thus, both the effective use of an acoustic nanomotor as an active transporter and the small payload needed for efficient gene knockout are intriguing results of the study.
«Gene knockout using new CRISPR tool makes mosquitoes highly resistant to malaria parasite: Study highlights the potential of mosquito gene - modification to combat malaria.»
An international team of researchers has identified a cause for chronic bad breath (halitosis), with the help of gene knockout mice from the UC Davis Mouse Biology Program.
Gene knockouts in people provide drug safety, effectiveness clues.
In our tests, the Gene Knockout Kit gave us greater than 80 percent knock - out rates for seven targets,» shares Shondra Miller, Ph.D., Director, Center for Advanced Genome Engineering at St. Jude Children's Research Hospital, on Synthego's web site.
Such method is called «Gene knockout.»
«Gene knockout: Loss of a gene can be compensated by another gene: Effects of genome interventions depend on the methods used.»
The research also demonstrated how these non-random outcomes can be harnessed to produce a desired effect, such as a gene knockout or the reading frame restoration of a disease - causing allele.
We use transgenes and gene knockouts to study regulatory processes in vivo.
In these mouse lines, gene knockout can be induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP - FRT.
Cas9 nucleases from Streptococcus pyogenes (SpCas9) have been widely used for both gene knockout and knock - in at the level of single or multiple genes.
Overall, making a human cell line with a gene knockout was straightforward, and is a testament to how far genome engineering has come.