Sentences with word «isotype»

Flow cytometric analysis (A) of MCF7, MCF7 / HER2, and BT474 cells labeled with F3 - IgG, trastuzumab, or a human IgG isotype control antibody.

A polyclonal antibody contains a mixture of several isotypes of immunoglobulin G (e.g., IgG1, IgG2a).

aureus IgG isotypes influences surface protein A mediated immune subversion.

In our hematopoietic project area we try to understand factors involved in the B cell switch to expression of specific immunoglobulin isotypes such as IgE, which is key to the allergic reaction.

«In fact, this antibody isotype class switch is also assumed as a critical step in the development of other autoimmune diseases.

Primary isolates that were incubated with identical concentrations of FITC - and PE - conjugated isotype standards were used to assess the level of background intensity.

IHC - P mouse tumor tissue (from lung) with human cell line injected, some muscle tissue attached as well sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF) Ab: 1 ug /...

Rat anti-mouse IFN - γ (XMG1.2, 0.5 mg), rat anti-mouse TNF - α (XT3.11, 1 mg), and rat IgG2a isotype control (2A3, 0.5 mg) were injected i.p. every third day beginning on day 2 postinfection (8).

(E) Mice were treated beginning day 2 postinfection, and every 3 d thereafter with isotype control, IFN - γ blockade only, TNF - α blockade only, or IFN - γ / TNF - α dual blockade following infection.

Included are representations of artworks and museum exhibits created by artists Oliver Ressler, Sayler / Morris, Dread Scott, Temporary Services, and others, original Isotype graphics drawn from the Museum's lexicon of «capitalisms,» and texts from Lucy Lippard, Lester K. Spence, T.J. Demos, Chantal Mouffe, McKenzie Wark and Kim Stanley Robinson, among others.

Included are representations of artworks and museum exhibits created by artists Oliver Ressler, Sayler / Morris, Dread Scott, Temporary Services, and others, original Isotype graphics drawn from the... go to book page >>

Secondary labeling was carried out using isotype matched Alexa - fluor labeled secondary antibodies (AF488, 568 and 594, all from Invitrogen Corp).

Cultured human embryonic lung - derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14715 (0.2 µg / ml) followed by an AlexaFluor ® 488 - conjugated - goat - anti-mouse IgG2a isotype specific secondary antibody (2 µg / ml).

«Linkers are stuck in the nanoparticle membrane and bind to a fixed region of any antibody of the same isotype.

However, these were all of the antibody isotype M (immunoglobulin M, IgM), the earliest antibody type elicited during an acute immune response.

AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron - specific class III b - tubulin isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».

Cytogenetic aberrations in multiple myeloma are associated with shifts in serum immunoglobulin isotypes distribution and levels

A monoclonal antibody contains a single isotype of immunoglobulin.

Corresponding isotype control, mouse IgG ‐ APC (R&D, #IC002A, 1:10) rat IgG - PE (ebioscience, # 12-4301-81, 1:100), was used.

After washing with PBS and blocking for 30 minutes (IHC / ICC Blocking Buffer - Low Protein; eBioscience), cells were incubated with anti - Oct ‑ 4 antibody (diluted 1:150; eBiosicence) or an IgG2a K isotype control (diluted 1:150; eBiosicence) for one hour.

ADCC / ADCP - enabling isotypes of a-TIGIT surrogate Ab show potent monotherapy efficacy in murine tumor models that correlates to increased T cell activation and reduced Treg infiltration of tumors.

They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate isotype control for 15 min in the dark at 4 °C.

Quadrants were set based on isotype - stained controls.

A non-specific isotype control antibody (human IgG1) showed no reactivity.

An Alexa Fluor ® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.

According to studies of healthy individuals in the United States, the Tg antibodies differ from those seen in Hashimoto's thyroididis patients, by being polyreactive, of lower affinity and of predominantly IgM isotype.

In addition, rabbits only have one isotype of IgG molecules with more disulphide bonds.

Rat anti-mouse ST2 - blocking Ab and mouse IgG1 isotype control Ab were provided by Amgen.

There are many different immunoglobulin isotypes and without L - aspartic acid many of these compounds can not be synthesised.

Red histogram represents equal quantity of isotype control.

«We therefore assume that this class switch of the antibody isotype may contribute to the pathogenesis of GBS,» explains Meyer Sauteur.

By contrast, the anti-GalC antibodies in the GBS patients were of the isotype IgG.

Use negative controls (unstained and isotype control antibodies) to determine gating of populations.

Negative staining and isotype controls are included to assess antibody staining relative to background during FACS analysis.

You will find a wide variety of isotypes, species and conjugates in our website, such as gold, biotin, Dylight and Alexa Fluor ® dyes.

Isotype control antibody (black line) was mouse IgG2b [PLPV219](ab91366, 2µg / 1x106 cells) used under the same conditions.

HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

The isotype of the primary antibody should be indicated on the product's datasheet.

An isotype control for the secondary antibody was used as a negative control in both samples.

The secondary antibody must be specific to the isotype class of the primary antibody (e.g., IgG or IgM)