Sentences with word «lysate»
Lysate is a scientific term that refers to the liquid portion obtained after breaking open cells, usually through a process called lysis. It contains the components of the cells, such as proteins and genetic material, which can be studied or used for various experiments in laboratories. Full definition
(I) Immunoblotting was performed using the indicated antibodies on whole - cell lysates from cells incubated or not with TRAIL.
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The SMN (human) Enzyme Immunometric Assay (EIA) kit is a complete kit for the quantitative determination of SMN protein in cell lysates of human origin.
Notably, analysis of cell lysates from ABL2 - depleted and ABL1 / ABL2 double - knockdown breast cancer cells showed a greater reduction of the phosphorylation of CrkL compared to cells with knockdown of ABL1 alone (Fig. 2K).
Using the kits, you can quickly and easily label proteins in lysates or purified proteins with near - infrared CF ® dyes before electrophoresis and blotting.
Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.
A total amount of 225 μg protein, pooled from five whole tumour protein lysates of mice with the same genotype (75 μg each), was used.
Serum antibody reactivity to B. burgdorferi lysate antigen was significantly lower in antibiotic - treated mice compared to saline - treated mice at 2 months after completion of treatment (Figure 2).
The Clinical Biomarkers Facility offers services for high - throughput and highly specific analyses of protein biomarker candidates in body fluids such as plasma, serum, cerebrospinal fluids etc. and cell and tissue lysates using molecular tools such as proximity extension and proximity ligation technologies (PEA and PLA) providing assays with high specificity and sensitivity in complex biological matrices.
But after a while, I realized that I understood the rabbit reticulocyte lysate system much better than anybody else in the entire world, because I had made every single mistake that was possible to make and learned from them.
Gilbertie, cited for her study of platelet lysate therapy for infectious arthritis,
In platelet lysate therapy, plasma - rich platelets are manipulated to rapidly release strong regenerative properties into the body, stimulating growth of damaged tissue.
The antibody was incubated under agitation with Protein G beads for 10 min, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation.
Aliquots of the hippocampal lysates prepared as described above were analyzed by western blot for BDNF.
Led Immunoassay development, as a bioassay quantification tool, and competitive - inhibition immunoassay using cellular lysates.
All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».
Precleared nuclear lysates were probed with antibodies against Nrf2 or Bach1; a third set was not probed (mock).
Normal translation of human adenovirus mRNA in cell - free lysates prepared from abortively as well as productively infected monkey cells
Western blot results confirmed that tumor lysates from TRPV1 / KO mice treated with capsaicin and TPA exhibited significantly higher levels of COX - 2 than TRPV1 / WT mice (Fig. 2B).
They typically show up on ingredient labels as «plankton extract» or «micrococcus lysate.»
The research, performed using recombinant protein from cell lysate isolated in vitro, also validates a methodology for characterizing large, multivalent membrane proteins in general.
Obtained from EBV - immortalised peripheral blood Mononuclear cells from HIV - seropositive individuals.This antibody is anti-p24, and reacts with HIV lysate In ELISA.
various lysates were subjected to SDS PAGE followed by western blot with 10837 -1-AP (OPTN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours
The concentration of protease inhibitor should be 1.5 — 2 times that of a typical lysate preparation protocol for Western blotting.
Live cell assays constructed with genetically encoded, fluorescent biosensors can provide significant advantages over endpoint assays measured in cell lysates because functional information about the timing and location of cellular responses can be monitored in cells that are relevant to disease.
Following the provided protocol, a portion of the RNA - containing lysate (5 — 10 µl) was hybridized at 54 degrees C overnight to RNA specific magnetic capture beads in the presence of blocking buffers, proteinase K and preordered mRNA probe sets specific for the genes of interest: HMOX1, Nrf2, Keap1, Bach1, and GAPDH.
Plasma or serum samples were diluted 1:50 and reacted separately to 150 ug of infected and uninfected cell lysates overnight at 4 °C after protein separation through 4 - 12 % polyacrylamide gels and transfer to nytran membranes, as previously described [35, 36].
Western blot analysis of whole - cell lysates demonstrated that ospC7 no longer synthesized OspC (Fig. 3).
Some customers have successfully used ab7260 on Zebrafish lysates; however we have conflicting data to suggest that not all batches will be suitable for work on Zebrafish.
Anti-HIF-1-alpha unpurified antibody (ab51608) reactivity with reduced Hep3B cell lysate after transient transfection of scrambled siRNA (lanes1 - 3 and 7 - 9) or HIF -1-alpha siRNA (lanes 4 - 6 and 10 - 12).
Five days later, the live, treated mice showed a significant reduction in total luminescent flux, and spleen lysates from them showed a 99 % reduction in luciferase expression compared to HIV - LucR infected but untreated controls.
Antibody titers against B. burgdorferi cN40 lysates were determined by enzyme - linked immunosorbent assay (ELISA).
Gilbertie's project will explore how platelet - rich plasma lysate may be used to fight joint infections that cause inflammation and osteoarthritis in horses.
Horseshoe crabs» blue blood — specifically the clotting agent Limulus Amoebocyte Lysate (LAL)-- can help detect bacteria like E. coli.
They share the key ingredient, bifida ferment lysate, a probiotic that is said to act like a DNA repairing agent.
Rat brain tissue lysate was subjected to SDS PAGE followed by western blot with 23449 -1-AP (UBQLN2 antibody) at a dilution of 1:800 incubated at room temperature for 1.5 hours.
A transmembrane form is the predominant product generated in wheat germ (WG) extracts, whereas a completely translocated (secretory) form is the major product synthesized in rabbit reticulocyte lysates (RRL).
Aβ 1 to 40 and 1 to 42 levels in hippocampal lysate were analyzed using the Aβ1 - 40 and Aβ1 - 42 ELISA kits from Invitrogen (# KHB3481 and # KHB3442, respectively).
Complementary fluorescence correlation spectroscopy measurements were used to determine the average size of the RNA polymerase complex in cellular lysates.