Sentences with phrase «of endothelial cells»

The most potent of these, known as effector memory T cells, are activated by a group of proteins known as human leukocyte antigens (HLAs) on the surface of endothelial cells lining the donated organ's blood vessels.

Their work has shown that Ataluren, an investigational drug already in clinical trials for other genetic diseases, can — in some cases, slow or even reverse the excess proliferation of endothelial cells in diseased lung arteries.

The constitutive and inducible expression of the endothelial cell adhesion molecules E-selectin, ICAM - 1, and VCAM - 1 was assessed by immunohistochemistry (Figs. 4, A — C) ⇓.

Yet, some lymphocytes, having mutated to become cancerous, are equipped with a specific surface marker, the JAM - C protein, also present on the surface of endothelial cells.

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

This is the case of the extremely selective inhibitor of endothelial cell proliferation TNP - 470, a synthetic analog of fumagillin with enhanced anti-angiogenic properties [22], [23].

MicroRNA - 24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin - a3 and regulating inflammatory responses.

More recently, we have started to investigate the role of arachidonic acid P450 epoxygenases in the control of endothelial cell function in vitro and tumorigenesis in vivo.

In the past 10 years our laboratory has investigated the role of cell - matrix interaction in the control of endothelial cell functions in vivo and in vitro and provide the first evidence that the collagen binding integrin alpha1beta1 is pro-angiogenic and pro-tumorigenic and its inhibition and / or down - regulation is beneficial in the setting of tumor associated angiogenesis.

The adaptor CRADD / RAIDD controls activation of endothelial cells by proinflammatory stimuli.

By having such a mechanism, it is possible to minimize the loss of endothelial cells over a lifetime, due to naturally occurring cell death.

This gave a mixed population of cells with 20 % expressing CD34, a hematopoietic stem cell marker, with some co-expression of endothelial cell markers (CD31, Flk1, and VE - cadherin).

Using a technique called phase contrast video microscopy, Ubogu's team produced videos that show AIDP patient leukocytes as they migrated across the layer of endothelial cells that form the blood - nerve barrier in the laboratory.

In contrast, lymphocytes in both types of endothelial cell grafts expressed higher levels of genes known to be involved in dampening the immune response and inducing self - tolerance.

Yet, more recent discoveries suggest a much more active role of endothelial cells during tumor growth than has been anticipated so far.

Then, in an in vitro model of vascular leak, the authors found that NS1 fractured the integrity of endothelial cell monolayers through a TLR4 - dependent pathway, a finding that was supported by the observation that a TLR4 antagonist quelled capillary leak in a mouse model of dengue virus infection.

We chose to evaluate the ability of each endothelial cell line to internalize DiI - Ac - LDL because this reagent has been used in the past as a selection agent to establish different endothelial cell lines (26), and the internalization of DiI - Ac - LDL is one of the listed specifications of commercially available endothelial cell lines.

This activated phenotype of endothelial cells may be regulated rather easily and cells directed toward a more differentiated state by making slight adjustments in the incubation temperature.

The constitutive expression of these endothelial cell adhesion molecules was very similar for most of the lines derived from different organs, i.e., very low expression levels of E-selectin and VCAM - 1 and more pronounced expression of ICAM - 1.

Cell proliferation analysis of endothelial cells growing in 10 % DMEM at 33 °C (permissive) conditions.

Established cell lines exhibited several inherent endothelial properties, including the expression of constitutive and inducible levels of endothelial cell adhesion molecules E-selectin, intercellular adhesion molecule - 1, and vascular cell adhesion molecule - 1, internalization of acetylated low - density lipoprotein, and formation of loop structures on Matrigel surfaces.

Obtaining pure populations of endothelial cells has been difficult because many of the markers used to distinguish endothelial cells are also expressed by a variety of other vascular cells (8).

A new study describes an improved protocol for the efficient differentiation of endothelial cells from pluripotent stem cell sources and highlights the importance of the MAPK and PI3K signaling pathways

VEGF normally promotes the growth of endothelial cells, which in turn helps build new blood vessels in tumors.

In vitro culture protocol that we demonstrate herein, allows for generation of endothelial cells sufficient to cover at least 200 cm2 area within two months from isolation (Figure 1E).

In this prospective, open and non-randomized study, the effect of alpha - lipoic acid on the progression of endothelial cell damage and the course of diabetic nephropathy, as assessed by measurement of plasma thrombomodulin and urinary albumin concentration (UAC), was evaluated in 84 patients with diabetes mellitus over 18 months.

Effect of Alpha - lipoic Acid on the Progression of Endothelial Cell Damage and Albuminuria in Patients with Diabetes Mellitus: An Exploratory Study Diabetes Res Clin Pract 2001 (Jun); 52 (3): 175 — 183 Oxidative stress plays a central role in the pathogenesis and progression of late microangiopathic complications (diabetic nephropathy) in diabetes mellitus.

We are exploring the possibility of a cell - based therapy to treat or cure hemophilia A. Current work is focused on the transplantation of a specialized subset of endothelial cells found in the liver: liver sinusoidal endothelial cells.

Because the interactions of endothelial cells with adjacent brain cells and factors secreted from these cells are necessary for BBB function, permeability of fluorescently tagged molecules was significantly reduced in the presence of factors from brain cells, modeling the of human BBB.

The scientists tracked the cytoskeletons of endothelial cells as they were crossed by immune cells in real time, the behavior of the nuclei of various white blood cells during active squeezing, and the fate of the various types of actin fibers that make up the endothelial cell skeletons.

Dismantling structural filaments within the cytoskeletons — the internal skeletons — of the endothelial cells creates the large holes, which can be several microns in diameter.

Sydenham chorea was like rheumatic fever of the brain, thought to occur when rheumatic fever progresses and strep antibodies (emboldened by fever) breaches the blood - brain barrier (BBB), the tight wall of endothelial cells ordinarily there to protect the brain from the outside world.

I had some research going in my lab on the mechanisms of endothelial cell migration that came to an abrupt halt for this reason.

In a collaborative effort between the Gladstone laboratories of Benoit Bruneau, PhD, Katherine Pollard, PhD, and Dr. Srivastava, the scientists used stem cell technology to make large amounts of endothelial cells from patients with CAVD, comparing them to healthy cells and mapping their genetic and epigenetic changes as they developed into valve cells.

A byproduct of HO - 1 is carbon monoxide, which promotes the survival of endothelial cells.

The Max Planck researchers then showed that less necroptosis of endothelial cells and less metastasis occur in genetically modified animals in which Death Receptor 6 is disabled.

Co-culture of endothelial cells (green) and pericytes (red) results in spheroids which are used to study the interactions of the two cell types in 3D.

An impermeable shield of endothelial cells that protects our brains from toxins and other threats that may lurk in circulating blood, the barrier can also exclude therapeutic drugs and, at times, essential biomolecules required for healthy brain development.

To determine inducible endothelial cell adhesion molecule expression (E-selectin, VCAM - 1, and ICAM - 1), some of the chambers were given medium containing 10 ng / ml TNF - α for 4 — 6 h (these time points were selected to correlate with the known kinetics of endothelial cell adhesion molecule expression; Refs.

This is the first evidence that starvation of endothelial cells could offer new therapeutic opportunities for the treatment of excessive angiogenesis in diseases (like cancer).

Activation of endothelial cells elicits a complex series of responses that include the elaboration of proteolytic enzymes, migration, and proliferation.

Many of the endothelial cell lines described here have been considered previously inaccessible for study of tumor angiogenesis, progression, and metastasis.

Efforts to study the molecular mechanisms that mediate these complex processes in different anatomical compartments have been impeded by difficulties in the isolation and propagation of endothelial cells from different organs.

We have observed on several occasions that, for the majority of endothelial cell lines, the transfer to 37 °C conditions for 72 h correlated with a 50 % reduction in cell proliferation, a value that continued to decrease with time.